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Axonal, perineurial regeneration and reconstitution of the blood-nerve-barrier following nerve grafting

Axonal, perineurial regeneration and reconstitution of the blood-nerve-barrier following nerve grafting
Axonal, perineurial regeneration and reconstitution of the blood-nerve-barrier following nerve grafting

Fibre contents and diameters of the highly myelinated normal aural nerve and the predominantly unmyelinated cervical sympathetic trunk of adult rate were studied by quantitative histological methods. Autogenous grafts of segments of the two types of nerve were inserted into the aural nerve in place of an excised piece. After 2 months, the regenerating myelinated fibre density was found to be significantly higher in the unmyelinated nerve grafts than in the myelinated grafts, while the mean diameter of their axons was less than in the comparable myelinated nerve graft. The regenerating cervical sympathetic trunk graft also showed a significant increase in the myelinated fibre. content when compared with the normal cervical sympathetic' trunk. Rural nerve autografts were performed on 1. intact rat aural nerves, 2. aural nerves which were isolated from their central connections proximal to the site of grafting. The effect of the presence and absence of regenerating axons upon reconstitution of the perineurium at the graft junction sites and upon the reestablishment of the blood nerve barrier to horseradish peroxidase were studied over the succeeding 3-48 weeks. Compartmentation of the nerve fascicle occurred at the graft junction site where the perineurium was damaged. Each compartment contained Schwann cells with or without axons and was surrounded by elongated fibroblast-like cells which resembled perineurial cells in the longer surviving animals. The result of this study suggests that a) compartments form in a nerve at the site of perineurial damage even in the absence of axons. b) Although compartmentation may be a mechanism for perineurial regeneration, blood vessels and the cell layers forming the compartments at the graft junction sites remain permeable to horseradish peroxidase for at least 12 months. c) The intact perineurium around the distal stump of an amputated nerve is permeable to horseradish peroxidase bu endoneurial blood vessels are not.

University of Southampton
Ahmed, Abdel Aziz Mahmoud
Ahmed, Abdel Aziz Mahmoud

Ahmed, Abdel Aziz Mahmoud (1980) Axonal, perineurial regeneration and reconstitution of the blood-nerve-barrier following nerve grafting. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

Fibre contents and diameters of the highly myelinated normal aural nerve and the predominantly unmyelinated cervical sympathetic trunk of adult rate were studied by quantitative histological methods. Autogenous grafts of segments of the two types of nerve were inserted into the aural nerve in place of an excised piece. After 2 months, the regenerating myelinated fibre density was found to be significantly higher in the unmyelinated nerve grafts than in the myelinated grafts, while the mean diameter of their axons was less than in the comparable myelinated nerve graft. The regenerating cervical sympathetic trunk graft also showed a significant increase in the myelinated fibre. content when compared with the normal cervical sympathetic' trunk. Rural nerve autografts were performed on 1. intact rat aural nerves, 2. aural nerves which were isolated from their central connections proximal to the site of grafting. The effect of the presence and absence of regenerating axons upon reconstitution of the perineurium at the graft junction sites and upon the reestablishment of the blood nerve barrier to horseradish peroxidase were studied over the succeeding 3-48 weeks. Compartmentation of the nerve fascicle occurred at the graft junction site where the perineurium was damaged. Each compartment contained Schwann cells with or without axons and was surrounded by elongated fibroblast-like cells which resembled perineurial cells in the longer surviving animals. The result of this study suggests that a) compartments form in a nerve at the site of perineurial damage even in the absence of axons. b) Although compartmentation may be a mechanism for perineurial regeneration, blood vessels and the cell layers forming the compartments at the graft junction sites remain permeable to horseradish peroxidase for at least 12 months. c) The intact perineurium around the distal stump of an amputated nerve is permeable to horseradish peroxidase bu endoneurial blood vessels are not.

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Published date: 1980

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Local EPrints ID: 462560
URI: http://eprints.soton.ac.uk/id/eprint/462560
PURE UUID: d4dd36e9-5b02-45bc-af70-93bbe597ed79

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Date deposited: 04 Jul 2022 19:22
Last modified: 04 Jul 2022 19:22

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Author: Abdel Aziz Mahmoud Ahmed

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