Insulin-like growth factor II in preovulatory follicules and ovarian cysts
Insulin-like growth factor II in preovulatory follicules and ovarian cysts
The thesis studied the origin of insulin-like growth factor-II (IGF-II) in human preovulatory follicles and ovarian cysts of IVF patients. Investigations were carried out to determine whether follicular IGF-II concentrations affected oocyte potential during in vitro fertilisation (IVF) treatment cycles.
A convenient and reproducible radioreceptor assay was developed for the measurement of IGF-II, using whole cells of the erythroleukaemia cell line K562. The assay was specific for IGF-II and showed no cross-reactivity with either IGF-I or insulin.
A new extraction method was developed for the removal of IGF binding proteins from samples prior to IGF assay. The technique combined acid/ethanol precipitation with Sep-pak C18 extraction. Recovery of IGFs was calculated for each sample extracted. The method was more convenient than column chromatography and several samples could be processed together. The procedure was verified with internal standards and the IGF-I concentrations measured in serum compared favourably with literature reports from groups which used the same antibody.
It was found that intrafollicular IGF-II concentrations were not useful as a marker to predict IVF oocyte outcome. There was a significant correlation between the serum and follicular concentrations of IGF-II and IGF-I, both of which were lower in follicular fluid than in serum. In addition the IGF-II:IGF-I ratio was preserved markedly between serum and follicular fluid of IVF patients in both natural and stimulated (buserelin/hMG) cycles. The data suggest that in the preovulatory follicle IGF-II concentrations are, like IGF-I, influenced primarily by serum concentrations.
University of Southampton
1993
Gadd, Stephanie Clare
(1993)
Insulin-like growth factor II in preovulatory follicules and ovarian cysts.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The thesis studied the origin of insulin-like growth factor-II (IGF-II) in human preovulatory follicles and ovarian cysts of IVF patients. Investigations were carried out to determine whether follicular IGF-II concentrations affected oocyte potential during in vitro fertilisation (IVF) treatment cycles.
A convenient and reproducible radioreceptor assay was developed for the measurement of IGF-II, using whole cells of the erythroleukaemia cell line K562. The assay was specific for IGF-II and showed no cross-reactivity with either IGF-I or insulin.
A new extraction method was developed for the removal of IGF binding proteins from samples prior to IGF assay. The technique combined acid/ethanol precipitation with Sep-pak C18 extraction. Recovery of IGFs was calculated for each sample extracted. The method was more convenient than column chromatography and several samples could be processed together. The procedure was verified with internal standards and the IGF-I concentrations measured in serum compared favourably with literature reports from groups which used the same antibody.
It was found that intrafollicular IGF-II concentrations were not useful as a marker to predict IVF oocyte outcome. There was a significant correlation between the serum and follicular concentrations of IGF-II and IGF-I, both of which were lower in follicular fluid than in serum. In addition the IGF-II:IGF-I ratio was preserved markedly between serum and follicular fluid of IVF patients in both natural and stimulated (buserelin/hMG) cycles. The data suggest that in the preovulatory follicle IGF-II concentrations are, like IGF-I, influenced primarily by serum concentrations.
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Published date: 1993
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Local EPrints ID: 462597
URI: http://eprints.soton.ac.uk/id/eprint/462597
PURE UUID: 5ace01f8-8e6e-48e1-9dfa-9ede9ca0370f
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Date deposited: 04 Jul 2022 19:30
Last modified: 04 Jul 2022 19:30
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Author:
Stephanie Clare Gadd
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