The measurement of adult plant resistance of wheat to yellow rust
The measurement of adult plant resistance of wheat to yellow rust
Existing methods of identifying previously unknown virulences ofPuccinia striiformis Westend., which causes yellow rust of wheat, on adult plants are time-consuming. Work was undertaken to reduce the time-element by developing a reliable monocyclio test in the glasshouse. The winter wheat cultivars chosen for study included Hobbit and Mans Huntsman, which are both popular commercial cultivars possessing different adult plant resistances. The isolates of the pathogen used included those which had overcome these resistances.A standard method of inoculation suitable for all growth stages was developed. A spore/talc mixture was blown onto plants on a turntable inside a chamber. A standard method of assessing spore production was developed. Spores were collected from infected leaves by auction via a cyclone head and weighed.The use of these methods to detect interactions between cultivars and isolates was evaluated in a series of glasshouse experiments. Results were compared with those from polythene tunnel tests. Some, but not all, adult plant reactions were expressed in the glasshouse experiments. Spore production was not found to give any additional information to percentage leaf area infection as a measure of disease severity. It was concluded that the glasshouse test needed further modification of inoculation timing and incubation conditions, before it could be used reliably to detect increased virulence in the pathogen population. Microscopic examination of infected seedling tissue showed no differences between spore germination and penetration of isolates on different cultivars. Some differences between development of infection hyphae from sub-stomatal vesicles were found, but they did not generally relate to adult plant infection levels. The ratio between spread of chlorosis and spread of mycelium from a point inoculation of plants at G.S.21+ was found to be related to adult plant compatibilities.
University of Southampton
1980
Walker, Valerie June
(1980)
The measurement of adult plant resistance of wheat to yellow rust.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Existing methods of identifying previously unknown virulences ofPuccinia striiformis Westend., which causes yellow rust of wheat, on adult plants are time-consuming. Work was undertaken to reduce the time-element by developing a reliable monocyclio test in the glasshouse. The winter wheat cultivars chosen for study included Hobbit and Mans Huntsman, which are both popular commercial cultivars possessing different adult plant resistances. The isolates of the pathogen used included those which had overcome these resistances.A standard method of inoculation suitable for all growth stages was developed. A spore/talc mixture was blown onto plants on a turntable inside a chamber. A standard method of assessing spore production was developed. Spores were collected from infected leaves by auction via a cyclone head and weighed.The use of these methods to detect interactions between cultivars and isolates was evaluated in a series of glasshouse experiments. Results were compared with those from polythene tunnel tests. Some, but not all, adult plant reactions were expressed in the glasshouse experiments. Spore production was not found to give any additional information to percentage leaf area infection as a measure of disease severity. It was concluded that the glasshouse test needed further modification of inoculation timing and incubation conditions, before it could be used reliably to detect increased virulence in the pathogen population. Microscopic examination of infected seedling tissue showed no differences between spore germination and penetration of isolates on different cultivars. Some differences between development of infection hyphae from sub-stomatal vesicles were found, but they did not generally relate to adult plant infection levels. The ratio between spread of chlorosis and spread of mycelium from a point inoculation of plants at G.S.21+ was found to be related to adult plant compatibilities.
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Published date: 1980
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Local EPrints ID: 462600
URI: http://eprints.soton.ac.uk/id/eprint/462600
PURE UUID: 34e23be9-85fb-4cd0-a796-b574f6fcd9d6
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Date deposited: 04 Jul 2022 19:30
Last modified: 04 Jul 2022 19:30
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Author:
Valerie June Walker
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