Three-dimensional reconstruction and electrotonic modelling of CA1 hippocampal neurones
Three-dimensional reconstruction and electrotonic modelling of CA1 hippocampal neurones
The relationship between the form and function of neurones has intrigued and frustrated neuroscientists almost since the invention of microscopy. Many attempts have been made to describe electrophysiological phenomena in terms of the dendritic branching structure, however, these have often been limited in the amount of morphological data that could be included. While models based on simplified geometries allow elegant analytical solutions and invaluable insight into neuronal function they cannot address the heterogeneity of real dendritic arborisations.
The measurement of complex dendritic structures requires the manipulation of large amounts of three-dimensional data. A system has been developed that allows neurones injected with a visible marker to be digitised and encoded into a useful database. This provides information about the morphological structure of the cell which can be used as the basis of a compartmental model.
Heuristic modelling of fully reconstructed neurones is a large numerical problem requiring efficient solution algorithms. A general purpose modelling program has been developed (MX), which it is hoped will prove a useful tool for electrophysiological modelling in the laboratory. MX has been rigorously tested, and applied to a database description of a CA1 hippocampal neurone in order to estimate its passive electrotonic properties by comparison of the simulated results to the electrophysiological recordings made from the original cell.
Electrophysiological recordings are restricted to the soma or major dendrites, remote from the site of synaptic input. MX has been used to assess the accuracy of voltage-clamp recordings of postsynaptic currents in CA1 cells. It has also been used to investigate the NMDA component of excitatory synaptic potentials in the kainate-lesioned hippocampus of temporal lobe epilepsy. Preliminary results are presented on multiple concurrent synaptic excitations.
University of Southampton
1993
Stockley, Edward William
(1993)
Three-dimensional reconstruction and electrotonic modelling of CA1 hippocampal neurones.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The relationship between the form and function of neurones has intrigued and frustrated neuroscientists almost since the invention of microscopy. Many attempts have been made to describe electrophysiological phenomena in terms of the dendritic branching structure, however, these have often been limited in the amount of morphological data that could be included. While models based on simplified geometries allow elegant analytical solutions and invaluable insight into neuronal function they cannot address the heterogeneity of real dendritic arborisations.
The measurement of complex dendritic structures requires the manipulation of large amounts of three-dimensional data. A system has been developed that allows neurones injected with a visible marker to be digitised and encoded into a useful database. This provides information about the morphological structure of the cell which can be used as the basis of a compartmental model.
Heuristic modelling of fully reconstructed neurones is a large numerical problem requiring efficient solution algorithms. A general purpose modelling program has been developed (MX), which it is hoped will prove a useful tool for electrophysiological modelling in the laboratory. MX has been rigorously tested, and applied to a database description of a CA1 hippocampal neurone in order to estimate its passive electrotonic properties by comparison of the simulated results to the electrophysiological recordings made from the original cell.
Electrophysiological recordings are restricted to the soma or major dendrites, remote from the site of synaptic input. MX has been used to assess the accuracy of voltage-clamp recordings of postsynaptic currents in CA1 cells. It has also been used to investigate the NMDA component of excitatory synaptic potentials in the kainate-lesioned hippocampus of temporal lobe epilepsy. Preliminary results are presented on multiple concurrent synaptic excitations.
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Published date: 1993
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Local EPrints ID: 462614
URI: http://eprints.soton.ac.uk/id/eprint/462614
PURE UUID: d9a772c4-ae6e-4f1a-86bd-ddf4ff9ad875
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Date deposited: 04 Jul 2022 19:31
Last modified: 04 Jul 2022 19:31
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Author:
Edward William Stockley
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