Investigations into the use of L'vov platforms in electrothermal atomisation-atomic absorption spectrometry for the determination of trace elements in body tissues and fluids
Investigations into the use of L'vov platforms in electrothermal atomisation-atomic absorption spectrometry for the determination of trace elements in body tissues and fluids
The development is described of a reliable, routine method for the determination of lead in whole blood, using atomic absorption spectrometry with electrothermal atomisation from a L'vov platform. Chemical treatment within the atomiser of in situ oxygen ashing followed by desorption of chemisorbed oxygen as carbon monoxide at 950oC eliminates matrix effects that could occur with the processing of large numbers of samples. The accuracy and precision of the blood lead method has been assessed over a continuous period of three years by: a) compliance with a strict internal quality control protocol; b) comparison with the established technique of micro-sampling flame atomic absorption spectrometry; c) participation in external quality assessment programmes. The L'vov platform has been confirmed as a simple means of improving atomisation conditions within an electrothermal atomiser. However, use of grooves in the side wall of the graphite tubes, to support the platform, produces variable rates of atomisation which results in gross day to day variation in atomic signals and analytical sensitivity. The relative degrees of electrical and conductive heating of a platform in either grooved or non-grooved graphite tubes has been assessed. Electrical heating of a L'vov platform in a grooved tube is approximately twelve times greater than in a non-grooved tube. Several alternative designs of L'vov platform have been compared under routine analytical conditions using the developed blood lead method. None of the designs performed as well as a conventional L'vov platform placed in a non-grooved tube. This combination appears to be the optimum configuration. Conclusions are drawn as to why none of these arrangements produce isothermal atomisation conditions. This study has shown that an integrated approach is required to atomiser chemistry. Application of the protococls developed in this study has enabled an improved method for the determination of manganese, in whole blood and serum, to be developed.
University of Southampton
1988
Shuttler, Ian Lawrence
(1988)
Investigations into the use of L'vov platforms in electrothermal atomisation-atomic absorption spectrometry for the determination of trace elements in body tissues and fluids.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The development is described of a reliable, routine method for the determination of lead in whole blood, using atomic absorption spectrometry with electrothermal atomisation from a L'vov platform. Chemical treatment within the atomiser of in situ oxygen ashing followed by desorption of chemisorbed oxygen as carbon monoxide at 950oC eliminates matrix effects that could occur with the processing of large numbers of samples. The accuracy and precision of the blood lead method has been assessed over a continuous period of three years by: a) compliance with a strict internal quality control protocol; b) comparison with the established technique of micro-sampling flame atomic absorption spectrometry; c) participation in external quality assessment programmes. The L'vov platform has been confirmed as a simple means of improving atomisation conditions within an electrothermal atomiser. However, use of grooves in the side wall of the graphite tubes, to support the platform, produces variable rates of atomisation which results in gross day to day variation in atomic signals and analytical sensitivity. The relative degrees of electrical and conductive heating of a platform in either grooved or non-grooved graphite tubes has been assessed. Electrical heating of a L'vov platform in a grooved tube is approximately twelve times greater than in a non-grooved tube. Several alternative designs of L'vov platform have been compared under routine analytical conditions using the developed blood lead method. None of the designs performed as well as a conventional L'vov platform placed in a non-grooved tube. This combination appears to be the optimum configuration. Conclusions are drawn as to why none of these arrangements produce isothermal atomisation conditions. This study has shown that an integrated approach is required to atomiser chemistry. Application of the protococls developed in this study has enabled an improved method for the determination of manganese, in whole blood and serum, to be developed.
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Published date: 1988
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Local EPrints ID: 462674
URI: http://eprints.soton.ac.uk/id/eprint/462674
PURE UUID: 643f0911-df86-452d-9d68-579c7d928156
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Date deposited: 04 Jul 2022 19:40
Last modified: 04 Jul 2022 19:40
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Author:
Ian Lawrence Shuttler
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