Electrophysiological studies of synaptic function in different regions of the hippocampus
Electrophysiological studies of synaptic function in different regions of the hippocampus
In vitro and in vivo electrophysiological techniques were used to study the differences of synaptic functions between the two poles of the hippoacampus. In in vitro studies, slices were cut in two orientations, transverse and longitudinal to the septo-temporal axis of the hippocampus. In these experiments paired pulse stimulation protocols were used to evaluate synaptic inhibition and potentiation protocols were used to evaluate synaptic inhibition and potentiation that contribute to both the normal and epileptiform behaviour of cells. In control in vitro studies a signficant difference in the level of paired pulse inhibition was found in the two regions, with very little inhibition in the ventral hippocampal tissue. Kainic acid (KA) produced changes in the activity of the CAl pyramidal cells in both acute and chronic preparations. The principle observation was that of a failure of synaptic inhibition which was most obvious in the septal pole. However in slices from hippocampi, chronically lesioned with KA, there was still a difference between the synapatic properties of each pole. This difference was not so obvious in the acute in vitro studies. Following KA treatment in both in vivo and in vitro preparations, bursts of population spikes i.e. epileptiform activity, remined most prominent in the ventral hippocampus. In control in vivo experiments paired pulse inhibition was more robust and no significant difference in recordings from each pole of the hippocampus was observed. It was particularly noticeable, in both in vivo and in vitro studies that the ventral pole of the hippocampus expressed less excitatory plasticity in the form of paired pulse potentiation than the dorsal region.
University of Southampton
1989
Radpour, Shahrzad
(1989)
Electrophysiological studies of synaptic function in different regions of the hippocampus.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
In vitro and in vivo electrophysiological techniques were used to study the differences of synaptic functions between the two poles of the hippoacampus. In in vitro studies, slices were cut in two orientations, transverse and longitudinal to the septo-temporal axis of the hippocampus. In these experiments paired pulse stimulation protocols were used to evaluate synaptic inhibition and potentiation protocols were used to evaluate synaptic inhibition and potentiation that contribute to both the normal and epileptiform behaviour of cells. In control in vitro studies a signficant difference in the level of paired pulse inhibition was found in the two regions, with very little inhibition in the ventral hippocampal tissue. Kainic acid (KA) produced changes in the activity of the CAl pyramidal cells in both acute and chronic preparations. The principle observation was that of a failure of synaptic inhibition which was most obvious in the septal pole. However in slices from hippocampi, chronically lesioned with KA, there was still a difference between the synapatic properties of each pole. This difference was not so obvious in the acute in vitro studies. Following KA treatment in both in vivo and in vitro preparations, bursts of population spikes i.e. epileptiform activity, remined most prominent in the ventral hippocampus. In control in vivo experiments paired pulse inhibition was more robust and no significant difference in recordings from each pole of the hippocampus was observed. It was particularly noticeable, in both in vivo and in vitro studies that the ventral pole of the hippocampus expressed less excitatory plasticity in the form of paired pulse potentiation than the dorsal region.
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Published date: 1989
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Local EPrints ID: 462702
URI: http://eprints.soton.ac.uk/id/eprint/462702
PURE UUID: f2c14160-f525-44c0-9890-30fdb658a399
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Date deposited: 04 Jul 2022 19:42
Last modified: 04 Jul 2022 19:42
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Author:
Shahrzad Radpour
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