An investigation into the measurement of plasma and intraplatelet platelet factor 4 and beta-thromboglobulin in health and thrombotic disease
An investigation into the measurement of plasma and intraplatelet platelet factor 4 and beta-thromboglobulin in health and thrombotic disease
An investigation has been made into ways of measuring plasma, urinary, intraplatelet platelet factor 4 and beta-thromboglobulin and platelet factor 4 mobilised following a heparin injection. The chosen methods were compared and used to assess platelet activation in health and disease, the platelet specificity of platelet factor 4 and beta-thromboglobulin and the measurement of a platelet-endothelial cell interaction. Normal ranges were established for all measurements. No effect of age, sex, weight, blood pressure, smoking, alcohol intake, diet, social class or diurnal rhythm was found for any of the measurements. An unacceptably high within person variation was found for the two urinary protein measurements. The tests were performed in patients with coronary artery disease, peripheral vascular disease, following cardiac valve replacement, in cerebro-vascular disease and in diabetes mellitus with and without atherosclerotic complications. Patients were also studied pre- and post surgery, in idiopathic thrombocytopaenic purpura, bone marrow aplasia, diffuse intravascular coagulation and myeloproliferative disease. Plasma and intraplatelet factor 4 and beta-thromboglobulin were significantly different to matched controls in the majority of patient groups studied. Notably, no differences were found in patients post a myocardial infarction. Multivariate analysis revealed plasma B-TG to be the best discriminator between patients and controls. The heparin-mobilisable platelet factor 4 was significantly higher than controls in only diabetics and female atherosclerotic patients. The lack of correlation between plasma, intraplatelet and heparin-mobilisable platelet proteins suggests different pathogeneses for their derivations and these are discussed together with the value and interpretation of these tests.
University of Southampton
Etherington, Michael Denis
6d5b1d4f-2c9c-4047-bef3-e38995ada4dc
1988
Etherington, Michael Denis
6d5b1d4f-2c9c-4047-bef3-e38995ada4dc
Etherington, Michael Denis
(1988)
An investigation into the measurement of plasma and intraplatelet platelet factor 4 and beta-thromboglobulin in health and thrombotic disease.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
An investigation has been made into ways of measuring plasma, urinary, intraplatelet platelet factor 4 and beta-thromboglobulin and platelet factor 4 mobilised following a heparin injection. The chosen methods were compared and used to assess platelet activation in health and disease, the platelet specificity of platelet factor 4 and beta-thromboglobulin and the measurement of a platelet-endothelial cell interaction. Normal ranges were established for all measurements. No effect of age, sex, weight, blood pressure, smoking, alcohol intake, diet, social class or diurnal rhythm was found for any of the measurements. An unacceptably high within person variation was found for the two urinary protein measurements. The tests were performed in patients with coronary artery disease, peripheral vascular disease, following cardiac valve replacement, in cerebro-vascular disease and in diabetes mellitus with and without atherosclerotic complications. Patients were also studied pre- and post surgery, in idiopathic thrombocytopaenic purpura, bone marrow aplasia, diffuse intravascular coagulation and myeloproliferative disease. Plasma and intraplatelet factor 4 and beta-thromboglobulin were significantly different to matched controls in the majority of patient groups studied. Notably, no differences were found in patients post a myocardial infarction. Multivariate analysis revealed plasma B-TG to be the best discriminator between patients and controls. The heparin-mobilisable platelet factor 4 was significantly higher than controls in only diabetics and female atherosclerotic patients. The lack of correlation between plasma, intraplatelet and heparin-mobilisable platelet proteins suggests different pathogeneses for their derivations and these are discussed together with the value and interpretation of these tests.
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Published date: 1988
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Local EPrints ID: 462706
URI: http://eprints.soton.ac.uk/id/eprint/462706
PURE UUID: 15dacfc9-73d0-42d8-ac8c-187d69befaff
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Date deposited: 04 Jul 2022 19:43
Last modified: 23 Jul 2022 01:08
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Author:
Michael Denis Etherington
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