Studies on virus-cell interactions : 1 RNA synthesis during picornavirus infection; 2 DNA polymerases or leukemic guinea pigs
Studies on virus-cell interactions : 1 RNA synthesis during picornavirus infection; 2 DNA polymerases or leukemic guinea pigs
The pattern of RNA synthesis in BMC virus infected mouse L cells, in the presence and absence of actinomycin D, was examined by polyacrylamide gel electrophoresis at various times post-infection. The viral genomic RNA was synthesised from at least (2-3) hours post-infection but synthesis. of 7s RNA, or other subgenomic RNA species was not detectable at any time. In addition, it was shown that BMC infection caused an inhibition of cellular RNA synthesis and, a degradation of preformed cellular RNA 'at late stages of the infectious cycle.Variable levels of sedimentable and non-sedimentable DNA polymerase activity were detected in plasma from L2C leukemic guinea pigs. The sedimentable DNA polymerise had some characteristics of an RNA directed DNA polymerase and it is suggested that this activity is due, at least in part, to a virus-associated reverse transcriptase. The soluble plasma DNA polymerase is distinct from the sedimentable enzyme but may be related to a DNA polymerase present in the nuclear fraction of leukemic guinea pig cells.All attempts to detect' reverse transcriptase in L2C cells, using various methods which bad been of value in related systems, were unsuccessful. As it seems probable that such an enzyme is present in these cells this failure is discussed in relation to possible contributing factors. During the attempts to detect intracellular RNA directed DNA polymerasethe DNA polymerases of whole cell extracts and extracts of the nuclear, soluble cytoplasmic, and microsomal subcellular fractions' were examined by chromatography oa phosphor and DEAE-cellulose. The template preferences of the five forms of DNA polymerase detected in this manner, were characterised and it is suggested that these five activities probably represent different intracellular locations of three distinct enzymes.
University of Southampton
1975
Hallinan, Francis Martin
(1975)
Studies on virus-cell interactions : 1 RNA synthesis during picornavirus infection; 2 DNA polymerases or leukemic guinea pigs.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The pattern of RNA synthesis in BMC virus infected mouse L cells, in the presence and absence of actinomycin D, was examined by polyacrylamide gel electrophoresis at various times post-infection. The viral genomic RNA was synthesised from at least (2-3) hours post-infection but synthesis. of 7s RNA, or other subgenomic RNA species was not detectable at any time. In addition, it was shown that BMC infection caused an inhibition of cellular RNA synthesis and, a degradation of preformed cellular RNA 'at late stages of the infectious cycle.Variable levels of sedimentable and non-sedimentable DNA polymerase activity were detected in plasma from L2C leukemic guinea pigs. The sedimentable DNA polymerise had some characteristics of an RNA directed DNA polymerase and it is suggested that this activity is due, at least in part, to a virus-associated reverse transcriptase. The soluble plasma DNA polymerase is distinct from the sedimentable enzyme but may be related to a DNA polymerase present in the nuclear fraction of leukemic guinea pig cells.All attempts to detect' reverse transcriptase in L2C cells, using various methods which bad been of value in related systems, were unsuccessful. As it seems probable that such an enzyme is present in these cells this failure is discussed in relation to possible contributing factors. During the attempts to detect intracellular RNA directed DNA polymerasethe DNA polymerases of whole cell extracts and extracts of the nuclear, soluble cytoplasmic, and microsomal subcellular fractions' were examined by chromatography oa phosphor and DEAE-cellulose. The template preferences of the five forms of DNA polymerase detected in this manner, were characterised and it is suggested that these five activities probably represent different intracellular locations of three distinct enzymes.
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Published date: 1975
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Local EPrints ID: 462713
URI: http://eprints.soton.ac.uk/id/eprint/462713
PURE UUID: d0c35eee-bbf0-46d7-8a9e-fe291b249c37
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Date deposited: 04 Jul 2022 19:43
Last modified: 04 Jul 2022 19:43
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Author:
Francis Martin Hallinan
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