Molecular biology of human enteric caliciviruses
Molecular biology of human enteric caliciviruses
At the start of this thesis the genomes of two SRSVs Southampton virus (SV) and Norwalk virus (NV) have been characterised. However, sequence comparison between two SRSVs and animal caliciviruses suggested that the published SV and NV genome sequences may be incomplete as they both lack conserved motifs at the 5' termini of their respective genomes. This led to a further attempt to define the SV genomic 5' terminal sequence using RT-PCR and homopolymer tailing. An additional sequence of 12 nucleotides was identified which significantly changed the genome organisation by extending the first large open reading frame (ORF) by 51 amino acids. The 5' terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the SV and the animal caliciviruses RHDV and FCV.
A genomic clone of SV was constructed, sequenced and used to study translation of the genomic RNA in vitro. Three major translation products of 113, 48 and 41 kDa were identified in a coupled transcription-translation system.
Classic human enteric caliciviruses (HuCVs) have a distinctive morphology and are primarily associated with paediatric acute gastro-enteritis. Although morphologically distinct from the small round structured viruses (SRSVs), the classic HuCVs are thought to be closely related and were anticipated to have a similar genome organisation. The complete genome sequence of a classic HuCV (Manchester virus) was determined. The RNA genome (7266 nt) is smaller than the genome of SRSVs from the two genetic groups and has a unique arrangement of open reading frames.
A full-length Manchester virus cDNA clone was constructed and polyprotein processing was investigated in vitro. The major translation products of approximately 38kDa and 28kDa were observed which could represent 2C helicase and N terminal peptide respectively.
University of Southampton
Liu, Binlei
40305b0c-4707-415b-91f4-c1705f65c222
1996
Liu, Binlei
40305b0c-4707-415b-91f4-c1705f65c222
Liu, Binlei
(1996)
Molecular biology of human enteric caliciviruses.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
At the start of this thesis the genomes of two SRSVs Southampton virus (SV) and Norwalk virus (NV) have been characterised. However, sequence comparison between two SRSVs and animal caliciviruses suggested that the published SV and NV genome sequences may be incomplete as they both lack conserved motifs at the 5' termini of their respective genomes. This led to a further attempt to define the SV genomic 5' terminal sequence using RT-PCR and homopolymer tailing. An additional sequence of 12 nucleotides was identified which significantly changed the genome organisation by extending the first large open reading frame (ORF) by 51 amino acids. The 5' terminal bases pGpT and the presence of conserved genome and putative subgenomic RNA terminal motifs are now prominent features shared between the SV and the animal caliciviruses RHDV and FCV.
A genomic clone of SV was constructed, sequenced and used to study translation of the genomic RNA in vitro. Three major translation products of 113, 48 and 41 kDa were identified in a coupled transcription-translation system.
Classic human enteric caliciviruses (HuCVs) have a distinctive morphology and are primarily associated with paediatric acute gastro-enteritis. Although morphologically distinct from the small round structured viruses (SRSVs), the classic HuCVs are thought to be closely related and were anticipated to have a similar genome organisation. The complete genome sequence of a classic HuCV (Manchester virus) was determined. The RNA genome (7266 nt) is smaller than the genome of SRSVs from the two genetic groups and has a unique arrangement of open reading frames.
A full-length Manchester virus cDNA clone was constructed and polyprotein processing was investigated in vitro. The major translation products of approximately 38kDa and 28kDa were observed which could represent 2C helicase and N terminal peptide respectively.
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Published date: 1996
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Local EPrints ID: 462971
URI: http://eprints.soton.ac.uk/id/eprint/462971
PURE UUID: 87afdd77-aacb-4b79-a5c6-dd2eeb64b2b2
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Date deposited: 04 Jul 2022 20:33
Last modified: 22 Feb 2023 18:55
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Author:
Binlei Liu
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