The developmental assembly of sensory axon arrays in the central nervous system of Drosophila melanogaster : a combined cellular, genetic and molecular study
The developmental assembly of sensory axon arrays in the central nervous system of Drosophila melanogaster : a combined cellular, genetic and molecular study
It has been shown that the p[GAL4] enhancer-trap and UAS-lacZ reporter gene expression system of Brand & Perrimon (1993) can be used to reliably visualise the central axonal projections of subgroups of sensory neurons in Drosophila. (Smith & Shepherd, 1996).
The aims of the work presented here were twofold. First, to identify p[GAL4] lines that reveal subsets of sensory axons in larval and pupal CNS and describe the normal order of the projections. Second, to look for defects in these projections in mutants, thus, identifying genes important for the establishment of the order.
From a screen of pupae of 159 p[GAL4] lines, 42 revealed sensory axon projections in the CNS. These lines served to identify new sensory neurons, confirm previous descriptions of the central projections of sensory axons and provide new descriptions of the axon projections from defined sensory neurons. Four lines showing lacZ expression patterns of interest (104Y, 275Y, S33, C787) were further examined at the neuroanatomical level and the visible sensory projections and expressing cells described. P-element mediated mutagenesis was performed and lethal excision deletions generated for three (275Ye6, S33e69, C787e8, e14, e18, e42, e55). All three were examined for their time of lethality and neurogenic or peripheral axon growth guidance defects in embryos. Each mutant line was crossed to a p[GAL4] line with an overlapping pattern of expression and the mutants screened for central defects. One first instar larva from 275Ye6 showed severe defects. Plasmid rescue was employed for lines S33 & C787 and 260 base pairs of genomic sequence obtained for C787. No homology with known base nucleotide sequences was obtained.
One line, C161, was used to mark proprioceptive axons in the CNS of disco mutants. This showed severe defects in larval and pupal CNS of mutants. A motility study confirmed the variable sensory defects were manifest by variable coordinary defects.
University of Southampton
Glossop, Nicholas Robert John
1997
Glossop, Nicholas Robert John
Glossop, Nicholas Robert John
(1997)
The developmental assembly of sensory axon arrays in the central nervous system of Drosophila melanogaster : a combined cellular, genetic and molecular study.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
It has been shown that the p[GAL4] enhancer-trap and UAS-lacZ reporter gene expression system of Brand & Perrimon (1993) can be used to reliably visualise the central axonal projections of subgroups of sensory neurons in Drosophila. (Smith & Shepherd, 1996).
The aims of the work presented here were twofold. First, to identify p[GAL4] lines that reveal subsets of sensory axons in larval and pupal CNS and describe the normal order of the projections. Second, to look for defects in these projections in mutants, thus, identifying genes important for the establishment of the order.
From a screen of pupae of 159 p[GAL4] lines, 42 revealed sensory axon projections in the CNS. These lines served to identify new sensory neurons, confirm previous descriptions of the central projections of sensory axons and provide new descriptions of the axon projections from defined sensory neurons. Four lines showing lacZ expression patterns of interest (104Y, 275Y, S33, C787) were further examined at the neuroanatomical level and the visible sensory projections and expressing cells described. P-element mediated mutagenesis was performed and lethal excision deletions generated for three (275Ye6, S33e69, C787e8, e14, e18, e42, e55). All three were examined for their time of lethality and neurogenic or peripheral axon growth guidance defects in embryos. Each mutant line was crossed to a p[GAL4] line with an overlapping pattern of expression and the mutants screened for central defects. One first instar larva from 275Ye6 showed severe defects. Plasmid rescue was employed for lines S33 & C787 and 260 base pairs of genomic sequence obtained for C787. No homology with known base nucleotide sequences was obtained.
One line, C161, was used to mark proprioceptive axons in the CNS of disco mutants. This showed severe defects in larval and pupal CNS of mutants. A motility study confirmed the variable sensory defects were manifest by variable coordinary defects.
This record has no associated files available for download.
More information
Published date: 1997
Identifiers
Local EPrints ID: 462992
URI: http://eprints.soton.ac.uk/id/eprint/462992
PURE UUID: 283a1667-1f75-4955-92ef-239ad1fabb83
Catalogue record
Date deposited: 04 Jul 2022 20:35
Last modified: 04 Jul 2022 20:35
Export record
Contributors
Author:
Nicholas Robert John Glossop
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics