New approaches to the diagnosis of malignant lymphomas
New approaches to the diagnosis of malignant lymphomas
New heat mediated antigen retrieval methods and new monoclonal antibodies have greatly enlarged the scope of immunohistochemistry using paraffin embedded tissue. The polymerase chain reaction (PCR) method may also be applied to paraffin embedded tissue and can detect T and B-cell monoclonality and chromosomal translocations. The aims of this thesis are to assess the diagnostic value of these methods by applying them to a series of lymphomas that can be difficult to diagnose using histology alone.
Microwave antigen retrieval is more effective than trypsin digestion for the demonstration of immunoglobulin light chains. Light chain restriction was demonstrated in 91% of B-cell lymphomas using the microwave method. Light chain immunohistochemistry and bcl-2 protein staining were the most helpful methods for distinguishing follicular lymphomas from reactive follicular hyperplasia. Light chain restriction was demonstrated in 83% and bcl-2 protein was positive in 98% of lymphomas. PCR was helpful in fewer cases, a monoclonal IgH rearrangement was detected in 44% and t(14;18) in 32% of lymphomas. Light chain restriction was demonstrated in 80% of cutaneous B-cell lymphomas and monoclonal IgH rearrangement was detected in 45%. A monoclonal TCRγ rearrangement was demonstrated in 59% of cases of established MF and 50% of borderline biopsies from patients with subsequent MF. Monoclonal TCRγ gene rearrangement was detected in 4 of 6 cases of EATL and at least one ulcer from all cases of UJ. These findings support the suggestion that UJ is a manifestation of EATL.
Molecular methods can assist in the diagnosis of malignant lymphomas. However, there is a high false negative rate so a negative result does not exclude lymphoma. Good light chain staining is more helpful than PCR for the diagnosis of B-cell lymphomas.
University of Southampton
Ashton-Key, Margaret
5111ac18-7d4f-4ef0-9c71-0a44c37aaed4
1997
Ashton-Key, Margaret
5111ac18-7d4f-4ef0-9c71-0a44c37aaed4
Ashton-Key, Margaret
(1997)
New approaches to the diagnosis of malignant lymphomas.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
New heat mediated antigen retrieval methods and new monoclonal antibodies have greatly enlarged the scope of immunohistochemistry using paraffin embedded tissue. The polymerase chain reaction (PCR) method may also be applied to paraffin embedded tissue and can detect T and B-cell monoclonality and chromosomal translocations. The aims of this thesis are to assess the diagnostic value of these methods by applying them to a series of lymphomas that can be difficult to diagnose using histology alone.
Microwave antigen retrieval is more effective than trypsin digestion for the demonstration of immunoglobulin light chains. Light chain restriction was demonstrated in 91% of B-cell lymphomas using the microwave method. Light chain immunohistochemistry and bcl-2 protein staining were the most helpful methods for distinguishing follicular lymphomas from reactive follicular hyperplasia. Light chain restriction was demonstrated in 83% and bcl-2 protein was positive in 98% of lymphomas. PCR was helpful in fewer cases, a monoclonal IgH rearrangement was detected in 44% and t(14;18) in 32% of lymphomas. Light chain restriction was demonstrated in 80% of cutaneous B-cell lymphomas and monoclonal IgH rearrangement was detected in 45%. A monoclonal TCRγ rearrangement was demonstrated in 59% of cases of established MF and 50% of borderline biopsies from patients with subsequent MF. Monoclonal TCRγ gene rearrangement was detected in 4 of 6 cases of EATL and at least one ulcer from all cases of UJ. These findings support the suggestion that UJ is a manifestation of EATL.
Molecular methods can assist in the diagnosis of malignant lymphomas. However, there is a high false negative rate so a negative result does not exclude lymphoma. Good light chain staining is more helpful than PCR for the diagnosis of B-cell lymphomas.
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Published date: 1997
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Local EPrints ID: 463043
URI: http://eprints.soton.ac.uk/id/eprint/463043
PURE UUID: 15cfa904-3137-473d-9261-7673da3a95d9
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Date deposited: 04 Jul 2022 20:40
Last modified: 04 Jul 2022 20:40
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Author:
Margaret Ashton-Key
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