Study of rice blast fungus Magnaporthe grisea (Hebert) of Bangladesh
Study of rice blast fungus Magnaporthe grisea (Hebert) of Bangladesh
Polymerase chain reaction (PCR) technology, based upon the random amplification of polymorphic DNA (RAPD) was used to assess the genetic variability among thirty nine isolate of M. grisea the causal organism of rice blast diseases, isolated from rice and three weed species from Bangladesh and a range of other countries. Sixty different DNA fragments of 0.2 to 2.8 kb were obtained using five 10-mer primers of arbitrary nucleotide sequences. Computer analysis of these data from five random primers showed a high genetic variability. The isolates that infect weed species Leersia hexendra were found to be genetically different from rice infecting and two other rice non-infecting isolates. In contrast isolates from weed species Digitaria setigera and Panicum repens shared a common RAPD profile produced by some of the rice infecting isolates.
The results suggest that the M. grisea populations infecting these two weed species may provide inoculum for rice blast disease in Bangladesh. Within these M. grisea isolates (weed and rice host) variation in the RAPD profiles did not appear to be related to origin, morphological characters or mating types. Further one band (approximately 0.79 kb), consistently appearing with rice infecting isolates when genomic DNA was amplified with primer (OPD-1), was used as a probe in Southern hybridizations. From the macroscopic symptom study, it was found that isolate Po28292 from weed species P. repens is nonpathogenic to rice. The L. hexendra weed host isolate Po29192 produced lesion type three on susceptible rice plants and is considered as a poor pathogen of rice. Poor pathogenicity is associated with reduced appressorium formation with longer germ tube and hypersensitive cell death. Further, the appressorium formation was not affected by the resistant or susceptible plant leaf surface.
University of Southampton
1998
Nahar, Niru Shamsun
(1998)
Study of rice blast fungus Magnaporthe grisea (Hebert) of Bangladesh.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Polymerase chain reaction (PCR) technology, based upon the random amplification of polymorphic DNA (RAPD) was used to assess the genetic variability among thirty nine isolate of M. grisea the causal organism of rice blast diseases, isolated from rice and three weed species from Bangladesh and a range of other countries. Sixty different DNA fragments of 0.2 to 2.8 kb were obtained using five 10-mer primers of arbitrary nucleotide sequences. Computer analysis of these data from five random primers showed a high genetic variability. The isolates that infect weed species Leersia hexendra were found to be genetically different from rice infecting and two other rice non-infecting isolates. In contrast isolates from weed species Digitaria setigera and Panicum repens shared a common RAPD profile produced by some of the rice infecting isolates.
The results suggest that the M. grisea populations infecting these two weed species may provide inoculum for rice blast disease in Bangladesh. Within these M. grisea isolates (weed and rice host) variation in the RAPD profiles did not appear to be related to origin, morphological characters or mating types. Further one band (approximately 0.79 kb), consistently appearing with rice infecting isolates when genomic DNA was amplified with primer (OPD-1), was used as a probe in Southern hybridizations. From the macroscopic symptom study, it was found that isolate Po28292 from weed species P. repens is nonpathogenic to rice. The L. hexendra weed host isolate Po29192 produced lesion type three on susceptible rice plants and is considered as a poor pathogen of rice. Poor pathogenicity is associated with reduced appressorium formation with longer germ tube and hypersensitive cell death. Further, the appressorium formation was not affected by the resistant or susceptible plant leaf surface.
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Published date: 1998
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Local EPrints ID: 463125
URI: http://eprints.soton.ac.uk/id/eprint/463125
PURE UUID: 12615454-dd15-4bc6-90bd-34876c530abc
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Date deposited: 04 Jul 2022 20:45
Last modified: 04 Jul 2022 20:45
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Author:
Niru Shamsun Nahar
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