The application of inductively coupled plasma source mass spectrometry to clinical biochemistry and environmental science
The application of inductively coupled plasma source mass spectrometry to clinical biochemistry and environmental science
A simple direct method was developed to determine Hg by ICP MS in diluted urine, diluted blood and in HNO3 digests of fish. Wash-out times of <130s with solutions of ≤30 μg 1-1 Hg were achieved by complexing Hg with (NH4)2H2EDTA and the presence of ammonia. An optional disinfection procedure for urine and whole blood utilised dilution with a virucidal agent, 'Virkon'. Virkon precipitated blood proteins, requiring subsequent dissolution using tetramethylammonium hydroxide. More than 85% of IQC results were within ± 1 μg 1-1 of their target values for blood and > 80% within 5% for urine. There was successful participation in 3 QA schemes. NIST (NBS) Albacore Tuna, gave 95% recovery of the target and good precision, 0.90 ± 0.01 μg g-1 (N=3). Detection limits (DLs) were 0.13 μg 1-1 in blood, 0.27 μg 1-1 in urine and 0.004 μg g-1 in fish.
A study of Hg exposure from gold mining in the Tapajos Valley, Brazil, found that a fishing village (JA), 100 km from mining activities, had higher blood Hg (mean 81 μg 1-1) (p<0.004) and a higher mean blood:urine ratio, 5.98 (p<0.001) than 3 other groups, related to fish consumption. Urine and blood Hg levels were related in the 2 mining groups (CR, p = 0.001; CU, P<0.001) but not the other 2 groups. There was higher exposure to organic Hg (from fish) by group JA, followed by group IT, a mining town.
Routine analysis at the Trace Element Unit, Southampton General Hospital, showed a blood Hg of ≤8 nmol l-1 was associated with the reporting of suspected symptoms of poisoning from amalgam fillings. There was evidence to support a reference value of < 10 nmol l-1 Hg in blood for individuals with no fish intake and <4 amalgam fillings. 206Pb.-207Pb ratio determination was used for source apportionment in 7 studies and 11 individual cases of Pb poisoning.
University of Southampton
1998
Moreton, Jennifer Anne
(1998)
The application of inductively coupled plasma source mass spectrometry to clinical biochemistry and environmental science.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
A simple direct method was developed to determine Hg by ICP MS in diluted urine, diluted blood and in HNO3 digests of fish. Wash-out times of <130s with solutions of ≤30 μg 1-1 Hg were achieved by complexing Hg with (NH4)2H2EDTA and the presence of ammonia. An optional disinfection procedure for urine and whole blood utilised dilution with a virucidal agent, 'Virkon'. Virkon precipitated blood proteins, requiring subsequent dissolution using tetramethylammonium hydroxide. More than 85% of IQC results were within ± 1 μg 1-1 of their target values for blood and > 80% within 5% for urine. There was successful participation in 3 QA schemes. NIST (NBS) Albacore Tuna, gave 95% recovery of the target and good precision, 0.90 ± 0.01 μg g-1 (N=3). Detection limits (DLs) were 0.13 μg 1-1 in blood, 0.27 μg 1-1 in urine and 0.004 μg g-1 in fish.
A study of Hg exposure from gold mining in the Tapajos Valley, Brazil, found that a fishing village (JA), 100 km from mining activities, had higher blood Hg (mean 81 μg 1-1) (p<0.004) and a higher mean blood:urine ratio, 5.98 (p<0.001) than 3 other groups, related to fish consumption. Urine and blood Hg levels were related in the 2 mining groups (CR, p = 0.001; CU, P<0.001) but not the other 2 groups. There was higher exposure to organic Hg (from fish) by group JA, followed by group IT, a mining town.
Routine analysis at the Trace Element Unit, Southampton General Hospital, showed a blood Hg of ≤8 nmol l-1 was associated with the reporting of suspected symptoms of poisoning from amalgam fillings. There was evidence to support a reference value of < 10 nmol l-1 Hg in blood for individuals with no fish intake and <4 amalgam fillings. 206Pb.-207Pb ratio determination was used for source apportionment in 7 studies and 11 individual cases of Pb poisoning.
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Published date: 1998
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Local EPrints ID: 463139
URI: http://eprints.soton.ac.uk/id/eprint/463139
PURE UUID: d662653c-1ab1-4539-9d87-ed22adb88bd5
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Date deposited: 04 Jul 2022 20:45
Last modified: 04 Jul 2022 20:45
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Author:
Jennifer Anne Moreton
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