Modulation of carbohydrate expression in the intestinal mucosa
Modulation of carbohydrate expression in the intestinal mucosa
Feeding a fibre-rich commercial diet to germ free rats increased the villus length and crypt depth but decreased the staining density of neutral and acidic carboxylated mucins in the small intestine. The same diet also increased the N-acetylglucosamine and sialic acid residues in the surface goblet cells of the small intestine and N-acetylgalactosamine residues in the surface goblet cells of the large intestine. This diet also reduced the number of endocrine cells in the germ free animals but increased the number of serotonin immunoreactive cells in the normal animals.
The presence of human flora increased the staining density of neutral and sulphomucins. The presence of rat flora reduced the sialic acid linked D-galactose residues and human flora specifically reduced the crypt-to-surface gradient of fucosyl expression. Both floras increased the serotonin immunoreactive cells in the colon. The use of lectin markers for mouse M-cells revealed that M-cells developing the dome epithelium of the reconstituted SCID mice. Mouse M cells were exclusively labelled by fucose-specific lectins but in human Peyer's patches no distinct M-cell staining pattern was observed.
A number of conclusions can be drawn from these studies. First, the diet-microbial interactions alter the mucosal architecture and the activity of enteroendocrine cells in the intestinal tract. Second, the dietary changes are influential in modifying the amount and proportion of mucin predominantly in the small intestine while the microbial flora influences the mucosal architecture predominately in the large intestine. Third, the lectins can be used to analyse any changes in the terminal carbohydrate residues of the mucus under different experimental conditions. Fourth, the use of bone marrow transplantation from normal Balb/c mice into SCID mice along with lectin markers for M-cells could be used to study the histogenesis of Peyer's patches. Fifth, the distinct differences in glycosylation between mouse and human Peyer's patches suggest that when considering cell surface gylcoconjugates as target molecules appropriate lectin should be chosen for each species.
University of Southampton
1999
Sharma, Ram Prakash
(1999)
Modulation of carbohydrate expression in the intestinal mucosa.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Feeding a fibre-rich commercial diet to germ free rats increased the villus length and crypt depth but decreased the staining density of neutral and acidic carboxylated mucins in the small intestine. The same diet also increased the N-acetylglucosamine and sialic acid residues in the surface goblet cells of the small intestine and N-acetylgalactosamine residues in the surface goblet cells of the large intestine. This diet also reduced the number of endocrine cells in the germ free animals but increased the number of serotonin immunoreactive cells in the normal animals.
The presence of human flora increased the staining density of neutral and sulphomucins. The presence of rat flora reduced the sialic acid linked D-galactose residues and human flora specifically reduced the crypt-to-surface gradient of fucosyl expression. Both floras increased the serotonin immunoreactive cells in the colon. The use of lectin markers for mouse M-cells revealed that M-cells developing the dome epithelium of the reconstituted SCID mice. Mouse M cells were exclusively labelled by fucose-specific lectins but in human Peyer's patches no distinct M-cell staining pattern was observed.
A number of conclusions can be drawn from these studies. First, the diet-microbial interactions alter the mucosal architecture and the activity of enteroendocrine cells in the intestinal tract. Second, the dietary changes are influential in modifying the amount and proportion of mucin predominantly in the small intestine while the microbial flora influences the mucosal architecture predominately in the large intestine. Third, the lectins can be used to analyse any changes in the terminal carbohydrate residues of the mucus under different experimental conditions. Fourth, the use of bone marrow transplantation from normal Balb/c mice into SCID mice along with lectin markers for M-cells could be used to study the histogenesis of Peyer's patches. Fifth, the distinct differences in glycosylation between mouse and human Peyer's patches suggest that when considering cell surface gylcoconjugates as target molecules appropriate lectin should be chosen for each species.
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Published date: 1999
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Local EPrints ID: 463628
URI: http://eprints.soton.ac.uk/id/eprint/463628
PURE UUID: 8468a506-7564-42ad-a663-009f7e24fcca
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Date deposited: 04 Jul 2022 20:54
Last modified: 04 Jul 2022 20:54
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Author:
Ram Prakash Sharma
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