Genetic diversity of Pummelo (Citrus grandis L.Osbeck) in Nepal and improvement of propagation methods
Genetic diversity of Pummelo (Citrus grandis L.Osbeck) in Nepal and improvement of propagation methods
Pummelo (Citrus grandis L. Osbeck) is one of the under-utilised fruit species with high potential for its commercialisation in south and south-east Asia. In this study attempts were made to assess the genetic diversity of pummelo, to select superior genotypes in Nepal and to develop appropriate propagation techniques for rapid cloning with the aim of distributing quality planting materials to farmers.
Farmers' participatory survey and diversity study were carried out in different agro-climatic regions of Nepal. A total of 132 pummelo trees were evaluated from 114 homestead gardens. Wide variation in yield efficiency, rind thickness, pulp colour, pulp and juice content, total soluble solids, total acid, seeds per fruit, and in shape and size of fruit, leaf and petiole wing were recorded. Cluster analysis on quantitative data produced 5 discrete groups. Diversity of four isozymes (esterase, alcohol dehydrogenase, glutamate oxaloacetate transaminase and acid phophatase) was evaluated in polyacrylamide gel to confirm the field observations. A total of 39 different genotypes were observed in 43 accessions of pummelo. Cluster analysis of isozyme data produced 7 groups. Farmers inputs were found very useful for the assessment of diversity and identification of superior trees. They preferred fruits with sweet, non-bitter, red colour and high pulp content as the varietal selection criteria. Based on these criteria 6 accessions were identified as superior genotypes for mid-hill and plain areas of Nepal.
Stem cutting, air-layering and six grafting methods were studied to identify suitable growth regulator(s), season, media and the methods of vegetative propagation for pummelo. Stem cuttings taken in the month of April from about one month old shoots and grown in sterilised soil:sand mixture under polythene tunnel produced 75 percent rooting without auxin treatment and 90% of the rooted cuttings survived during hardening.
University of Southampton
1999
Paudyal, Krishna Prasad
(1999)
Genetic diversity of Pummelo (Citrus grandis L.Osbeck) in Nepal and improvement of propagation methods.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Pummelo (Citrus grandis L. Osbeck) is one of the under-utilised fruit species with high potential for its commercialisation in south and south-east Asia. In this study attempts were made to assess the genetic diversity of pummelo, to select superior genotypes in Nepal and to develop appropriate propagation techniques for rapid cloning with the aim of distributing quality planting materials to farmers.
Farmers' participatory survey and diversity study were carried out in different agro-climatic regions of Nepal. A total of 132 pummelo trees were evaluated from 114 homestead gardens. Wide variation in yield efficiency, rind thickness, pulp colour, pulp and juice content, total soluble solids, total acid, seeds per fruit, and in shape and size of fruit, leaf and petiole wing were recorded. Cluster analysis on quantitative data produced 5 discrete groups. Diversity of four isozymes (esterase, alcohol dehydrogenase, glutamate oxaloacetate transaminase and acid phophatase) was evaluated in polyacrylamide gel to confirm the field observations. A total of 39 different genotypes were observed in 43 accessions of pummelo. Cluster analysis of isozyme data produced 7 groups. Farmers inputs were found very useful for the assessment of diversity and identification of superior trees. They preferred fruits with sweet, non-bitter, red colour and high pulp content as the varietal selection criteria. Based on these criteria 6 accessions were identified as superior genotypes for mid-hill and plain areas of Nepal.
Stem cutting, air-layering and six grafting methods were studied to identify suitable growth regulator(s), season, media and the methods of vegetative propagation for pummelo. Stem cuttings taken in the month of April from about one month old shoots and grown in sterilised soil:sand mixture under polythene tunnel produced 75 percent rooting without auxin treatment and 90% of the rooted cuttings survived during hardening.
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Published date: 1999
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Local EPrints ID: 463633
URI: http://eprints.soton.ac.uk/id/eprint/463633
PURE UUID: b8158c9b-753a-4d01-bc05-84b8784144b8
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Date deposited: 04 Jul 2022 20:54
Last modified: 04 Jul 2022 20:54
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Author:
Krishna Prasad Paudyal
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