Aspects of the employment of fish to study gene expression and of the effects of the release of transgenic fish
Aspects of the employment of fish to study gene expression and of the effects of the release of transgenic fish
The development and potential applications of transgenic fish are described. The expression of a ubiquitin C-terminal hydrolase enzyme (UCHL-1/PGP 9.5) was studied in a range of tissues from three fish species (the zebrafish (Danio rerio), the nile tilapia (Oreochromis niloticus) and the rainbow trout (Oncorhynchus mykiss)) by western blotting. One of two mouse monoclonal antibodies detected a fish homologue of PGP 9.5 as did a rabbit polyclonal antiserum. The antibodies used were also employed on tissue sections of larval zebrafish and tilapia.
The debate surrounding of the possible environmental effects of the release of transgenic fish is reviewed.
Two trials of transgenic fish of two different species (common carp Cyprinus carpio and channel catfish Ictalurus punctatus) in outdoor ponds in the United Stated are described in detail in order to illustrate the types of confinement measures which may be required to prevent the escape of transgenic fish.
Mathematical models are employed to produce estimates of the likely change of frequency of transgenic fish introduced into a population of their conspecifics. Both directional and stabilizing selection (heterozygote advantage) are considered. The effects of different magnitude and direction (transgene advantageous or deleterious) of selection acting on the transgene, degree of dominance of the transgene and the initial frequency and genotype (heterozygous/hemizygous versus homozygous) of the introduced transgenic fish are evaluated.
Later models incorporate additional factors including repeated introductions of transgenic fish, differential selection in the two sexes, sex-linked (non-autosomal) transgenes and multiple transgene loci. The effects of assortative mating are summarized in the main body of the text and described in detail in appendix 1. The complexities of extending the model to incorporate overlapping generations are also described. The range of situations and parameters modelled is significantly wider than any described in the literature published to date, whether for fish or any other transgenic animal.
University of Southampton
1998
Laight, Richard James
(1998)
Aspects of the employment of fish to study gene expression and of the effects of the release of transgenic fish.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The development and potential applications of transgenic fish are described. The expression of a ubiquitin C-terminal hydrolase enzyme (UCHL-1/PGP 9.5) was studied in a range of tissues from three fish species (the zebrafish (Danio rerio), the nile tilapia (Oreochromis niloticus) and the rainbow trout (Oncorhynchus mykiss)) by western blotting. One of two mouse monoclonal antibodies detected a fish homologue of PGP 9.5 as did a rabbit polyclonal antiserum. The antibodies used were also employed on tissue sections of larval zebrafish and tilapia.
The debate surrounding of the possible environmental effects of the release of transgenic fish is reviewed.
Two trials of transgenic fish of two different species (common carp Cyprinus carpio and channel catfish Ictalurus punctatus) in outdoor ponds in the United Stated are described in detail in order to illustrate the types of confinement measures which may be required to prevent the escape of transgenic fish.
Mathematical models are employed to produce estimates of the likely change of frequency of transgenic fish introduced into a population of their conspecifics. Both directional and stabilizing selection (heterozygote advantage) are considered. The effects of different magnitude and direction (transgene advantageous or deleterious) of selection acting on the transgene, degree of dominance of the transgene and the initial frequency and genotype (heterozygous/hemizygous versus homozygous) of the introduced transgenic fish are evaluated.
Later models incorporate additional factors including repeated introductions of transgenic fish, differential selection in the two sexes, sex-linked (non-autosomal) transgenes and multiple transgene loci. The effects of assortative mating are summarized in the main body of the text and described in detail in appendix 1. The complexities of extending the model to incorporate overlapping generations are also described. The range of situations and parameters modelled is significantly wider than any described in the literature published to date, whether for fish or any other transgenic animal.
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Published date: 1998
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Local EPrints ID: 463643
URI: http://eprints.soton.ac.uk/id/eprint/463643
PURE UUID: dab48c75-32ec-4364-8f6d-b564f204c558
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Date deposited: 04 Jul 2022 20:54
Last modified: 04 Jul 2022 20:54
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Author:
Richard James Laight
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