In vitro propagation of egyptian date palm (Phoenix Dactylifera L.) cultivars Zaghloul and Samani
In vitro propagation of egyptian date palm (Phoenix Dactylifera L.) cultivars Zaghloul and Samani
An in vitro propagation system has been developed for an important data palm (Phoenix dactylifera L.) cv Zaghloul grown in the Egyptian mid delta region. In addition, another date palm cv Samani was evaluated for in vitro propagation. Shoot tip explants were taken from offshoots of mature palms and were cultured either whole or as sections as MS medium. Contamination and browning hindered establishment of long-term cultures. Various combinations of growth regulators were tested to induce callus growth and minimise browning.
The two cultivars differed in their response with cv Zaghloul being more responsive. Picloram was particularly effective for inducing callus. Browning was less evident with shoot tip sections than with intact shoot tips. Addition of various concentrations of glycine and glutamine individually promoted callus formation and enhanced the formation of embryogenic callus. The addition of various concentrations of thiamine, nicotinic acid and pyridoxine individually stimulated callus growth with cv Zaghloul but not with cv Samani. These vitamins did not enhance the formation of embryogenic callus with cv Zaghloul and only had a marginal effect with cv Samani.
Plantlets were recovered from embryogenic callus but those of cv Samania were weak and did not survive through rooting. Zaghloul plantlets derived from somatic embryos were cultured on different concentrations of NAA and IBA with or without charcoal treatments to evaluate their effect on rooting, and survival in the free-living conditions. Culture on polyethylene glycol supplemented media before transfer to the greenhouse, increased survival. Timing of transfer of plants from laboratory to greenhouse was also a factor in plant survival. Plants were successfully established in soil. RAPDs was used to evaluate the genetic composition of the plants recovered and some variants were identified, although morphologically they appeared identical to the non-variants.
University of Southampton
1999
El Shiaty, Olfat Hamed
(1999)
In vitro propagation of egyptian date palm (Phoenix Dactylifera L.) cultivars Zaghloul and Samani.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
An in vitro propagation system has been developed for an important data palm (Phoenix dactylifera L.) cv Zaghloul grown in the Egyptian mid delta region. In addition, another date palm cv Samani was evaluated for in vitro propagation. Shoot tip explants were taken from offshoots of mature palms and were cultured either whole or as sections as MS medium. Contamination and browning hindered establishment of long-term cultures. Various combinations of growth regulators were tested to induce callus growth and minimise browning.
The two cultivars differed in their response with cv Zaghloul being more responsive. Picloram was particularly effective for inducing callus. Browning was less evident with shoot tip sections than with intact shoot tips. Addition of various concentrations of glycine and glutamine individually promoted callus formation and enhanced the formation of embryogenic callus. The addition of various concentrations of thiamine, nicotinic acid and pyridoxine individually stimulated callus growth with cv Zaghloul but not with cv Samani. These vitamins did not enhance the formation of embryogenic callus with cv Zaghloul and only had a marginal effect with cv Samani.
Plantlets were recovered from embryogenic callus but those of cv Samania were weak and did not survive through rooting. Zaghloul plantlets derived from somatic embryos were cultured on different concentrations of NAA and IBA with or without charcoal treatments to evaluate their effect on rooting, and survival in the free-living conditions. Culture on polyethylene glycol supplemented media before transfer to the greenhouse, increased survival. Timing of transfer of plants from laboratory to greenhouse was also a factor in plant survival. Plants were successfully established in soil. RAPDs was used to evaluate the genetic composition of the plants recovered and some variants were identified, although morphologically they appeared identical to the non-variants.
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Published date: 1999
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Local EPrints ID: 463753
URI: http://eprints.soton.ac.uk/id/eprint/463753
PURE UUID: 28700507-f75d-47c1-83cc-50c2709147ab
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Date deposited: 04 Jul 2022 20:56
Last modified: 04 Jul 2022 20:56
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Author:
Olfat Hamed El Shiaty
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