Rat brain acetylcholin-esterase : regional activity studies following various behavioural treatments

Abstract

The activity of acetYlcholinesterase, a stable enzyme and a relatively easily measurable component of the cholinergic transmission system, has been investigated in twelve regions of the adult rat brain following at various behavioural treatments. The aim of the StV has been to determine the nature and degree of changes of the enzyme activity following the treatments* For this purposet treatments have been chosen which incorporate a wide spectrum of physiological stimulation. Automated enzyme assay procedures were developed and used to assay the enzyme activity in females, only, of an inbred strain of Agus albino rat. The activity of acety1cholinesterase changes as a result of the behavioural treatments in five of the twelve brain regions. In four of these five the enzyme activity changes are directly related to the state of activation of the animals while, in the other regionp the enzyme activity changes"are of a different nature. Enzyme - kinetic studies on the altered enzyme indicate that the activity changes are a result of an increased availability of active sites and are not due to changes in the affinity of the enzyme for its substrate. Time - course studies indicate that the enzyme activity changes persist for several days* Protein synthesis inhibition studies are being conducted to investigate the mechanism of the observed enzyme activity changes* Other possible mechanisms are considered.

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