Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry
Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5’-alkyne and the other with a 3’-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
6859-6864
Kumar, Ravindra
dfd53d15-f740-4a18-90ad-efa6f2c5e488
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Tumpane, John
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Lincoln, Per
f1edcf40-411d-4f54-bd94-d13394c011d4
Wilhelmsson, L. Marcus
bcf4f67a-198c-4c5e-80b8-a78f743509d4
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
May 2007
Kumar, Ravindra
dfd53d15-f740-4a18-90ad-efa6f2c5e488
El-Sagheer, Afaf
05b8295a-64ad-4fdf-ad57-c34934a46c04
Tumpane, John
b8edc11a-1930-4b53-bdd3-8fd6456be05b
Lincoln, Per
f1edcf40-411d-4f54-bd94-d13394c011d4
Wilhelmsson, L. Marcus
bcf4f67a-198c-4c5e-80b8-a78f743509d4
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kumar, Ravindra, El-Sagheer, Afaf, Tumpane, John, Lincoln, Per, Wilhelmsson, L. Marcus and Brown, Tom
(2007)
Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.
Journal of the American Chemical Society, 129 (21), .
(doi:10.1021/ja070273v).
Abstract
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5’-alkyne and the other with a 3’-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
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Published date: May 2007
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Local EPrints ID: 46412
URI: http://eprints.soton.ac.uk/id/eprint/46412
ISSN: 0002-7863
PURE UUID: 99377d09-f69e-4de0-8ea7-94de4319826b
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Date deposited: 27 Jun 2007
Last modified: 16 Mar 2024 03:48
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Contributors
Author:
Ravindra Kumar
Author:
Afaf El-Sagheer
Author:
John Tumpane
Author:
Per Lincoln
Author:
L. Marcus Wilhelmsson
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