ICAM-1 and CD44 expression in human bronchial epithelium and the role of CD44 isoforms in cell adhesion, migration, and repair\dShih-hsing Leir.
ICAM-1 and CD44 expression in human bronchial epithelium and the role of CD44 isoforms in cell adhesion, migration, and repair\dShih-hsing Leir.
Mechanical damage of confluent epithelial cells induced the expression of CD44 on the cells up to 500 μm from the wound edge and for up to 48 hours. Before cell confluence, the expression of CD44 at low cell densities was significantly higher than in confluent cultures, while ICAM-1 was lower. IFNγ and TNFα co-stimulation increased ICAM-1 expression significantly, while little change was seen in CD44. IL-1β and IL-4 induced the expression of CD44s, CD44v3 and CD44v9. Individual primary epithelial cells expressed several CD44 isoforms, while CD44 isoforms were undetectable in columnar epithelial cells from human airway. Down-regulation of v8-v9-v10 isoforms and small decreases of v6-v7-v8-v9-v10 and a v3-containing isoforms were seen in the cells with cytokine treatments and mechanical damage. In addition, the function of CD44 was investigated in cell adhesion and migration. Cytokines induced a CD44s-dependent cell adhesion to hyaluronic acid (HA). IFNγ-induced cell binding to HA without increasing the level of cell surface CD44 indicated that other mechanisms are involved in the modulation of CD44/HA binding. CD44 antibodies inhibited cell migration and demonstrated that CD44 plays an important role in cell migration, and may be associated with the repair processes of bronchial epithelium. I have found increased CD44 protein expression or changes in the alternative splicing of CD44 isoforms during the repair of epithelial damage. This study describes the sub-cellular expression and regulation of ICMA-1 and CD44, and provides some indication of functions of these CAMs in human bronchial epithelial cells.
University of Southampton
Leir, Shih-Hsing
c534558c-19b3-409c-afcc-fedc66e1e3e5
2000
Leir, Shih-Hsing
c534558c-19b3-409c-afcc-fedc66e1e3e5
Leir, Shih-Hsing
(2000)
ICAM-1 and CD44 expression in human bronchial epithelium and the role of CD44 isoforms in cell adhesion, migration, and repair\dShih-hsing Leir.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Mechanical damage of confluent epithelial cells induced the expression of CD44 on the cells up to 500 μm from the wound edge and for up to 48 hours. Before cell confluence, the expression of CD44 at low cell densities was significantly higher than in confluent cultures, while ICAM-1 was lower. IFNγ and TNFα co-stimulation increased ICAM-1 expression significantly, while little change was seen in CD44. IL-1β and IL-4 induced the expression of CD44s, CD44v3 and CD44v9. Individual primary epithelial cells expressed several CD44 isoforms, while CD44 isoforms were undetectable in columnar epithelial cells from human airway. Down-regulation of v8-v9-v10 isoforms and small decreases of v6-v7-v8-v9-v10 and a v3-containing isoforms were seen in the cells with cytokine treatments and mechanical damage. In addition, the function of CD44 was investigated in cell adhesion and migration. Cytokines induced a CD44s-dependent cell adhesion to hyaluronic acid (HA). IFNγ-induced cell binding to HA without increasing the level of cell surface CD44 indicated that other mechanisms are involved in the modulation of CD44/HA binding. CD44 antibodies inhibited cell migration and demonstrated that CD44 plays an important role in cell migration, and may be associated with the repair processes of bronchial epithelium. I have found increased CD44 protein expression or changes in the alternative splicing of CD44 isoforms during the repair of epithelial damage. This study describes the sub-cellular expression and regulation of ICMA-1 and CD44, and provides some indication of functions of these CAMs in human bronchial epithelial cells.
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Published date: 2000
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Local EPrints ID: 464127
URI: http://eprints.soton.ac.uk/id/eprint/464127
PURE UUID: 97c1209c-5a6a-4277-a687-59b73c153c0b
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Date deposited: 04 Jul 2022 21:19
Last modified: 16 Mar 2024 19:16
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Shih-Hsing Leir
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