The University of Southampton
University of Southampton Institutional Repository

Development of techniques for wide hybridization in the genus cicer l.

Development of techniques for wide hybridization in the genus cicer l.
Development of techniques for wide hybridization in the genus cicer l.

Cross compatibility between three Cicer L. species viz. C. arietinum, C. reticulatum and C. pinnatifidum was investigated using a wide range of parental genotypes. The species were reciprocally cross compatible. However, isolating mechanism due to post-fertilization barriers existed between the species. The isolating mechanisms resulted in zygote mortality, embryo inviability and hybrid seedling lethality. Reciprocal differences were noticed among interspecific crosses in relation to pod set and development to maturity, the stage of embryo development and abortion, and hybrid plant viability.

A comparative study of early embryo development was carried out in the species and their reciprocal crosses. Embryo and endosperm development were normal in the parental species. In the crosses, embryo failure at very early stages was noticed resulting in pod abortion between 9-13 DAP. This was due to disharmonious interaction between embryo, suspensor, endosperm, and maternal tissues. Growth regulators were applied to enhance pod development and prevent early embryo regulators were applied to enhance pod development and prevent early embryo abortion. However, it was found that growth regulator application was not as effective in encouraging hybrid embryo development as it was in pod growth. The attempts to produce hybrids using embryo culture techniques was unfortunately unsuccessful, probably due to the fact that embryo abortion had started at an early stage. An in vitro system was developed for culture of young embryos of parental species. This may be used in production of hybrids when it is possible to excise the embryos and culture them in vitro.

University of Southampton
Taheri, Zhila
d6c351c4-3cde-41d1-a1bb-69e9aedff6df
Taheri, Zhila
d6c351c4-3cde-41d1-a1bb-69e9aedff6df

Taheri, Zhila (2000) Development of techniques for wide hybridization in the genus cicer l. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

Cross compatibility between three Cicer L. species viz. C. arietinum, C. reticulatum and C. pinnatifidum was investigated using a wide range of parental genotypes. The species were reciprocally cross compatible. However, isolating mechanism due to post-fertilization barriers existed between the species. The isolating mechanisms resulted in zygote mortality, embryo inviability and hybrid seedling lethality. Reciprocal differences were noticed among interspecific crosses in relation to pod set and development to maturity, the stage of embryo development and abortion, and hybrid plant viability.

A comparative study of early embryo development was carried out in the species and their reciprocal crosses. Embryo and endosperm development were normal in the parental species. In the crosses, embryo failure at very early stages was noticed resulting in pod abortion between 9-13 DAP. This was due to disharmonious interaction between embryo, suspensor, endosperm, and maternal tissues. Growth regulators were applied to enhance pod development and prevent early embryo regulators were applied to enhance pod development and prevent early embryo abortion. However, it was found that growth regulator application was not as effective in encouraging hybrid embryo development as it was in pod growth. The attempts to produce hybrids using embryo culture techniques was unfortunately unsuccessful, probably due to the fact that embryo abortion had started at an early stage. An in vitro system was developed for culture of young embryos of parental species. This may be used in production of hybrids when it is possible to excise the embryos and culture them in vitro.

Text
755848.pdf - Version of Record
Available under License University of Southampton Thesis Licence.
Download (28MB)

More information

Published date: 2000

Identifiers

Local EPrints ID: 464169
URI: http://eprints.soton.ac.uk/id/eprint/464169
PURE UUID: 2998afcd-d12a-4c9c-ba67-8433a87024f3

Catalogue record

Date deposited: 04 Jul 2022 21:23
Last modified: 16 Mar 2024 19:18

Export record

Contributors

Author: Zhila Taheri

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×