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The modulatory effects of dietary sulphur amino acids, tryptophan and arginine in young growing rats indergoing an inflammatory response

The modulatory effects of dietary sulphur amino acids, tryptophan and arginine in young growing rats indergoing an inflammatory response
The modulatory effects of dietary sulphur amino acids, tryptophan and arginine in young growing rats indergoing an inflammatory response

The extent to which dietary intervention, by correcting deficiencies in sulphur amino acids, tryptophan, and arginine intake, improves antioxidant defences and modulates the metabolic response to infection was examined in this thesis using a young rat model. Rats were given an inflammatory challenge by intraperitoneal injection of endotoxin, and were compared with ad libitum and pair-fed controls.

Glutathione (GSH) concentrations in various organs (liver, lung, spleen and thymus), and hepatic protein content decreased in animals fed the low-protein diets. In endotoxin-treated rats, the magnitude of the increase of the acute phase protein, α-1 acid glycoprotein, was reduced in animals fed the low-protein diets.

Addition of the sulphur amino acid, methionine, to the low-protein diets, restored GSH concentrations and increased hepatic protein content in endotoxin-treated animals and in animals fed ad libitum. In endotoxin-treated rats, addition of methionine to the low-protein diets reduced (not statistically significant) the number of neutrophils in lung, while in ad libitum and in pair-fed animals, addition of methionine increased (not statistically significant) the number of neutrophils. Furthermore, before starting treatments, addition of methionine to the low-protein diets improved growth.

In general, there was no indication that addition of tryptophan or arginine had marked effects on the acute inflammatory response after exposure to the endotoxin.

To conclude, dietary methionine is the most important amino acid regarding the ability to modulate the inflammatory response.

University of Southampton
Alhamdan, Adel Abdualwahab Hamdan
7104fe89-4492-4db5-8d10-82ec7220f06b
Alhamdan, Adel Abdualwahab Hamdan
7104fe89-4492-4db5-8d10-82ec7220f06b

Alhamdan, Adel Abdualwahab Hamdan (2000) The modulatory effects of dietary sulphur amino acids, tryptophan and arginine in young growing rats indergoing an inflammatory response. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

The extent to which dietary intervention, by correcting deficiencies in sulphur amino acids, tryptophan, and arginine intake, improves antioxidant defences and modulates the metabolic response to infection was examined in this thesis using a young rat model. Rats were given an inflammatory challenge by intraperitoneal injection of endotoxin, and were compared with ad libitum and pair-fed controls.

Glutathione (GSH) concentrations in various organs (liver, lung, spleen and thymus), and hepatic protein content decreased in animals fed the low-protein diets. In endotoxin-treated rats, the magnitude of the increase of the acute phase protein, α-1 acid glycoprotein, was reduced in animals fed the low-protein diets.

Addition of the sulphur amino acid, methionine, to the low-protein diets, restored GSH concentrations and increased hepatic protein content in endotoxin-treated animals and in animals fed ad libitum. In endotoxin-treated rats, addition of methionine to the low-protein diets reduced (not statistically significant) the number of neutrophils in lung, while in ad libitum and in pair-fed animals, addition of methionine increased (not statistically significant) the number of neutrophils. Furthermore, before starting treatments, addition of methionine to the low-protein diets improved growth.

In general, there was no indication that addition of tryptophan or arginine had marked effects on the acute inflammatory response after exposure to the endotoxin.

To conclude, dietary methionine is the most important amino acid regarding the ability to modulate the inflammatory response.

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Published date: 2000

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Local EPrints ID: 464231
URI: http://eprints.soton.ac.uk/id/eprint/464231
PURE UUID: 6d40a630-f643-49b4-825a-2d6ab819b257

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Date deposited: 04 Jul 2022 21:41
Last modified: 16 Mar 2024 19:21

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Author: Adel Abdualwahab Hamdan Alhamdan

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