The Use of Raman Spectroscopy in Pharmaceutical Analysis
The Use of Raman Spectroscopy in Pharmaceutical Analysis
In this thesis, the use of Raman spectroscopy in pharmaceutical analysis is evaluated. Sample rotation in FT-Raman spectroscopy is studied. Sample rotation is particularly important when analysing heat sensitive samples such as polymorphs. By rotating the sample, laser heating by the near infrared laser is reduced. However, modulation and double modulation in the FT-instrument might induce spurious lines into the Raman spectrum. Therefore it is essential that rotational speeds are kept reasonably low. Another reason to rotate samples is to avoid sub sampling. Especially when performing quantitative analysis, this is of major concern.
An attempt is made to further develop FT-Raman spectroscopy into a quantitative technique with the aim to make it applicable for analysis of pharmaceutical tablets. A study into FT-Raman intensity reproducibility of both liquids and solids is performed. Subsequently different sets of tablets were prepared and the correlation between concentration and Raman intensity evaluated. In addition, a comparison between internal and external standardisation is made.
In another chapter, a study into a local anaesthetic system is performed. Vibrational spectroscopy is used to assess the extent of interactions between the two active ingredients in the formulation. In addition, interactions between the two actives and an aqueous solution of two block copolymers, in which they are present, are evaluated. On increasing the temperature, this aqueous solution of block copolymers goes into the gel state. Therefore a start into the study of conformation changes of the aqueous solution of block copolymers alone and combined with the two active components is made.
A last chapter deals with the polymorphism of sulfathiazole. Sulfathiazole, an antibacterial agent, can exist in five different polymorphic forms. Polymorph I is thought to display unusual large anisotropic lattice expansion. This phenomenon is studied by vibrational spectroscopy and an attempt is made to correlate the NH stretching frequencies with the available X-ray data.
University of Southampton
De Paepe, Anne TG
153c685f-6c8f-44e0-b813-71423c9161f3
2001
De Paepe, Anne TG
153c685f-6c8f-44e0-b813-71423c9161f3
De Paepe, Anne TG
(2001)
The Use of Raman Spectroscopy in Pharmaceutical Analysis.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
In this thesis, the use of Raman spectroscopy in pharmaceutical analysis is evaluated. Sample rotation in FT-Raman spectroscopy is studied. Sample rotation is particularly important when analysing heat sensitive samples such as polymorphs. By rotating the sample, laser heating by the near infrared laser is reduced. However, modulation and double modulation in the FT-instrument might induce spurious lines into the Raman spectrum. Therefore it is essential that rotational speeds are kept reasonably low. Another reason to rotate samples is to avoid sub sampling. Especially when performing quantitative analysis, this is of major concern.
An attempt is made to further develop FT-Raman spectroscopy into a quantitative technique with the aim to make it applicable for analysis of pharmaceutical tablets. A study into FT-Raman intensity reproducibility of both liquids and solids is performed. Subsequently different sets of tablets were prepared and the correlation between concentration and Raman intensity evaluated. In addition, a comparison between internal and external standardisation is made.
In another chapter, a study into a local anaesthetic system is performed. Vibrational spectroscopy is used to assess the extent of interactions between the two active ingredients in the formulation. In addition, interactions between the two actives and an aqueous solution of two block copolymers, in which they are present, are evaluated. On increasing the temperature, this aqueous solution of block copolymers goes into the gel state. Therefore a start into the study of conformation changes of the aqueous solution of block copolymers alone and combined with the two active components is made.
A last chapter deals with the polymorphism of sulfathiazole. Sulfathiazole, an antibacterial agent, can exist in five different polymorphic forms. Polymorph I is thought to display unusual large anisotropic lattice expansion. This phenomenon is studied by vibrational spectroscopy and an attempt is made to correlate the NH stretching frequencies with the available X-ray data.
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Published date: 2001
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Local EPrints ID: 464495
URI: http://eprints.soton.ac.uk/id/eprint/464495
PURE UUID: d4071532-ed37-48e2-8287-92835adb8f6e
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Date deposited: 04 Jul 2022 23:41
Last modified: 16 Mar 2024 19:33
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Author:
Anne TG De Paepe
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