Sensory processing in the Isolated in vitro Rat Spinal Cord with particular emphasis on Opioid-related Peptides, Excitatory and Inhibitiory Amino Acids
Sensory processing in the Isolated in vitro Rat Spinal Cord with particular emphasis on Opioid-related Peptides, Excitatory and Inhibitiory Amino Acids
Using an in vitro preparation of the rat spinal cord, this study investigates possible roles for NPFF, Nociceptin, and excitatory and inhibitory amino acids in nociceptive processes in the dorsal horn. 2-3 hours following dissection, the in vitro spinal cord develops spontaneous dorsal root activity (SDA) that can be recorded from the dorsal roots.
Investigations were made into the role of excitatory and inhibitory amino acids in SDA and dorsal horn field potentials. DHFPs were evoked and recorded at 10 times thresholds. The effect of the GABAA antagonist bicuculline, glycine antagonist, strychnine, AMPA/Kainate antagonist CNQX and NMDA antagonist MK801 were examined on SDA. All compounds reversibly reduced SDA. Bicuculline, strychnine and CNQX were investigated on fast waves. Bicuculline and strychnine reversibly depressed S2 of the fast wave component, while having no significant effect on S1 or the slow wave. CNQX depressed components of DHFPs. It was confirmed from these data that GABAA receptors play a large role in SDA and PAD development. In addition to this glycine, NMDA and AMPA/Kainate receptors are also important, which has not been previously demonstrated in this isolated rat spinal cord preparation.
The roles of nociceptin, nociceptin (1-13), nociceptin (1-7), acetyl nociceptin, nocistatin and acetyl nocistatin were investigated on SDA.
Experiments described in this study verify the in vitro spinal cord as a reliable means for investigating the pharmacology of PAD and nociceptive processing in the dorsal horn. The results show that GABA, glycine and glutamate are major components in SDA. Nociceptin and NPFF clearly have a legitimate function in controlling inhibitory and excitatory processes in the spinal cord that contributes to generation of SDA and PAD.
University of Southampton
Maile, Rebecca Ann
2dbf33c1-a186-4d5f-928f-ac313f7cb37e
2002
Maile, Rebecca Ann
2dbf33c1-a186-4d5f-928f-ac313f7cb37e
Maile, Rebecca Ann
(2002)
Sensory processing in the Isolated in vitro Rat Spinal Cord with particular emphasis on Opioid-related Peptides, Excitatory and Inhibitiory Amino Acids.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Using an in vitro preparation of the rat spinal cord, this study investigates possible roles for NPFF, Nociceptin, and excitatory and inhibitory amino acids in nociceptive processes in the dorsal horn. 2-3 hours following dissection, the in vitro spinal cord develops spontaneous dorsal root activity (SDA) that can be recorded from the dorsal roots.
Investigations were made into the role of excitatory and inhibitory amino acids in SDA and dorsal horn field potentials. DHFPs were evoked and recorded at 10 times thresholds. The effect of the GABAA antagonist bicuculline, glycine antagonist, strychnine, AMPA/Kainate antagonist CNQX and NMDA antagonist MK801 were examined on SDA. All compounds reversibly reduced SDA. Bicuculline, strychnine and CNQX were investigated on fast waves. Bicuculline and strychnine reversibly depressed S2 of the fast wave component, while having no significant effect on S1 or the slow wave. CNQX depressed components of DHFPs. It was confirmed from these data that GABAA receptors play a large role in SDA and PAD development. In addition to this glycine, NMDA and AMPA/Kainate receptors are also important, which has not been previously demonstrated in this isolated rat spinal cord preparation.
The roles of nociceptin, nociceptin (1-13), nociceptin (1-7), acetyl nociceptin, nocistatin and acetyl nocistatin were investigated on SDA.
Experiments described in this study verify the in vitro spinal cord as a reliable means for investigating the pharmacology of PAD and nociceptive processing in the dorsal horn. The results show that GABA, glycine and glutamate are major components in SDA. Nociceptin and NPFF clearly have a legitimate function in controlling inhibitory and excitatory processes in the spinal cord that contributes to generation of SDA and PAD.
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Published date: 2002
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Local EPrints ID: 464758
URI: http://eprints.soton.ac.uk/id/eprint/464758
PURE UUID: 0397c167-8057-4fa1-99af-f80501e846d1
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Date deposited: 05 Jul 2022 00:00
Last modified: 16 Mar 2024 19:43
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Author:
Rebecca Ann Maile
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