The University of Southampton
University of Southampton Institutional Repository

Towards synthetic phage libraries

Towards synthetic phage libraries
Towards synthetic phage libraries

A phage is essentially made up of a single-strand of DNA and a protein coat, which displays multivalently a peptide that is encoded for by the single strand of DNA.  The first part of this thesis describes the synthesis and screening of multivalent peptide dendrimer conjugates in order to discover if “peptide dendrimer conjugates could be utilised, as a possible way to increase the affinity of the weak binding peptide ligands to their integrin receptors.  In the same way that this had been achieved with multivalent carbohydrate ligands?”   This question was addressed in chapters 2 and 3.

Chapter 2 describes the solid-phase synthesis of dendrimers for the preparation of multivalent ligand scaffolds.  Chapter 3 describes the synthesis of multivalent peptide-dendrimer conjugates and their subsequent screening in an ELISA.  A multivalent competitive binding molecule for the α4β1 integrin was found which was 12-fold more potent than its monovalent form.

In the second part of the thesis the aim was to synthesise a synthetic phage, comprising of a peptide sequence displayed on a multivalent scaffold coupled to an oligonucleotide sequence (upon which PCR could be performed), encoding the peptide sequence.  Chapter 4 describes the synthesis of a scaffold, which could facilitate the synthesis of multivalent ligand libraries (synthetic phage libraries).  The synthesis and screening of unsymmetrical peptide-dendrimer conjugates on this scaffold are also described. Chapter 5 describes the development of a synthetic phage as well as the screening methodology used to detect the binding of a synthetic phage to an integrin receptor.

University of Southampton
Monaghan, Seán Oliver
4c326a52-0694-4046-af18-dce01cdcb387
Monaghan, Seán Oliver
4c326a52-0694-4046-af18-dce01cdcb387

Monaghan, Seán Oliver (2002) Towards synthetic phage libraries. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

A phage is essentially made up of a single-strand of DNA and a protein coat, which displays multivalently a peptide that is encoded for by the single strand of DNA.  The first part of this thesis describes the synthesis and screening of multivalent peptide dendrimer conjugates in order to discover if “peptide dendrimer conjugates could be utilised, as a possible way to increase the affinity of the weak binding peptide ligands to their integrin receptors.  In the same way that this had been achieved with multivalent carbohydrate ligands?”   This question was addressed in chapters 2 and 3.

Chapter 2 describes the solid-phase synthesis of dendrimers for the preparation of multivalent ligand scaffolds.  Chapter 3 describes the synthesis of multivalent peptide-dendrimer conjugates and their subsequent screening in an ELISA.  A multivalent competitive binding molecule for the α4β1 integrin was found which was 12-fold more potent than its monovalent form.

In the second part of the thesis the aim was to synthesise a synthetic phage, comprising of a peptide sequence displayed on a multivalent scaffold coupled to an oligonucleotide sequence (upon which PCR could be performed), encoding the peptide sequence.  Chapter 4 describes the synthesis of a scaffold, which could facilitate the synthesis of multivalent ligand libraries (synthetic phage libraries).  The synthesis and screening of unsymmetrical peptide-dendrimer conjugates on this scaffold are also described. Chapter 5 describes the development of a synthetic phage as well as the screening methodology used to detect the binding of a synthetic phage to an integrin receptor.

Text
885299.pdf - Version of Record
Available under License University of Southampton Thesis Licence.
Download (24MB)

More information

Published date: 2002

Identifiers

Local EPrints ID: 464847
URI: http://eprints.soton.ac.uk/id/eprint/464847
PURE UUID: 46922d64-a2d3-4811-9726-4b2e58bd7e32

Catalogue record

Date deposited: 05 Jul 2022 00:04
Last modified: 16 Mar 2024 19:47

Export record

Contributors

Author: Seán Oliver Monaghan

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×