Invertebrate gametes as integrators of mutagen exposure in the marine environment
Invertebrate gametes as integrators of mutagen exposure in the marine environment
The main objective of this study was to investigate the potential for the gametes of Pomatoceros lamarckii to integrate genotoxic damage, thereby providing a potentially sensitive assay for studying the transfer of genetic damage between generations. A secondary aim was the development of sensitive and ecologically relevant bioassays that can be used for laboratory and environmental testing purposes. This tube-dwelling, rocky-shore polycheate was a suitable candidate species due to its wide geographic distribution, ease of collection and maintenance, sessile, filter-feeding habit, and lack of gametogenic synchrony, which thus provided a source of mature gametes for testing purposes throughout most of the year.
Chapter 4 describes the development and application of the anaphase aberration (AA) test to identify both aneugenic and clastogenic effects in cells from early embryos following in vitro (post-spawning) and in vivo (pre-spawning) exposure of oocytes. In an attempt to refine and aid interpretation of the AA findings, fluorescence in situ hybridisation (FISH) was adapted for use with interphase nuclei from embryo/larval stages, which showed a high incidence of aneuploidy following chemical exposure (Chapter 5). In an attempt to determine the male contribution of genetic damage, and also whether this was transferable to the offspring, Chapter 6 describes the use of the comet assay to measure DNA strand breaks in spermatozoa following pre- and post-spawning exposures. Chapter 7 summarises the findings, including highlighting the advantages and disadvantages of the different assays used in this study. The Discussion also sets out a rationale behind eco-genotoxicity testing focusing on the likely consequences as this relates to marine invertebrate populations. It is concluded that effects on reproductive stages (reduced fertilisation, abnormal gametes/offspring) may be more important in fitness terms than somatic cell mutation.
University of Southampton
Wilson, James Thomas
221a6ddb-ea5b-47c4-9c8b-d20d9481ca8f
2002
Wilson, James Thomas
221a6ddb-ea5b-47c4-9c8b-d20d9481ca8f
Wilson, James Thomas
(2002)
Invertebrate gametes as integrators of mutagen exposure in the marine environment.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The main objective of this study was to investigate the potential for the gametes of Pomatoceros lamarckii to integrate genotoxic damage, thereby providing a potentially sensitive assay for studying the transfer of genetic damage between generations. A secondary aim was the development of sensitive and ecologically relevant bioassays that can be used for laboratory and environmental testing purposes. This tube-dwelling, rocky-shore polycheate was a suitable candidate species due to its wide geographic distribution, ease of collection and maintenance, sessile, filter-feeding habit, and lack of gametogenic synchrony, which thus provided a source of mature gametes for testing purposes throughout most of the year.
Chapter 4 describes the development and application of the anaphase aberration (AA) test to identify both aneugenic and clastogenic effects in cells from early embryos following in vitro (post-spawning) and in vivo (pre-spawning) exposure of oocytes. In an attempt to refine and aid interpretation of the AA findings, fluorescence in situ hybridisation (FISH) was adapted for use with interphase nuclei from embryo/larval stages, which showed a high incidence of aneuploidy following chemical exposure (Chapter 5). In an attempt to determine the male contribution of genetic damage, and also whether this was transferable to the offspring, Chapter 6 describes the use of the comet assay to measure DNA strand breaks in spermatozoa following pre- and post-spawning exposures. Chapter 7 summarises the findings, including highlighting the advantages and disadvantages of the different assays used in this study. The Discussion also sets out a rationale behind eco-genotoxicity testing focusing on the likely consequences as this relates to marine invertebrate populations. It is concluded that effects on reproductive stages (reduced fertilisation, abnormal gametes/offspring) may be more important in fitness terms than somatic cell mutation.
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Published date: 2002
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Local EPrints ID: 464899
URI: http://eprints.soton.ac.uk/id/eprint/464899
PURE UUID: 585022a8-c8d5-49b7-8395-eb26bbb75325
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Date deposited: 05 Jul 2022 00:08
Last modified: 23 Jul 2022 02:14
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Author:
James Thomas Wilson
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