The University of Southampton
University of Southampton Institutional Repository

Investigation of the CD4+ T lymphocyte responses to Hepatitis C virus infection : cytokine production and its relationship to fibrosis and inflammation

Investigation of the CD4+ T lymphocyte responses to Hepatitis C virus infection : cytokine production and its relationship to fibrosis and inflammation
Investigation of the CD4+ T lymphocyte responses to Hepatitis C virus infection : cytokine production and its relationship to fibrosis and inflammation

This study investigated the phenotype and magnitude of the CD4+ T lymphocyte response to HCV to see if there was any correlation with the severity of the resultant liver disease. In addition, genetic markers which could influence the CD4+ T lymphocyte response were examined for correlation with severity of liver disease. Finally, the effect of the HCV specific CD4+ T lymphocyte response on the effector cells of hepatic fibrosis, hepatic stellate cells, was investigated.

Genomic DNA from CHC patients was tested for the presence of the allele HLA DQB1*0301, a Major Histocompatibility Complex class II molecule which has been shown to be over-represented in individuals with evidence of spontaneous resolution of acute infection. There was no correlation between frequency of this allele in our population and severity of CHC related liver disease. However, analysis of clinical information gathered from these patients confirmed that age, duration of infection and excess alcohol intake were all risk factors for more severe liver disease.  In addition, severity of fibrosis correlated positively with severity of inflammation; supporting the hypothesis that fibrosis is the result of chronic inflammation of CHC.

Functional studies of the CD4+ T lymphocyte response in HCV infection confirmed that HCV lymphocyte proliferative responses are different between CHC patients and those with evidence of spontaneous resolution of HCV infection. There were no differences in the HCV specific proliferative responses between groups of CHC patients discordant for severity of liver disease. However, HCV specific Interferon-γ secretion was more commonly seen in patients with severe liver disease.  CD4+ T lymphocyte responses to non-HCV recall antigens were identical between all groups.

University of Southampton
Brooks, Corinne
d9c02bb8-b1b1-4f98-a66e-a47a67f324d4
Brooks, Corinne
d9c02bb8-b1b1-4f98-a66e-a47a67f324d4

Brooks, Corinne (2002) Investigation of the CD4+ T lymphocyte responses to Hepatitis C virus infection : cytokine production and its relationship to fibrosis and inflammation. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

This study investigated the phenotype and magnitude of the CD4+ T lymphocyte response to HCV to see if there was any correlation with the severity of the resultant liver disease. In addition, genetic markers which could influence the CD4+ T lymphocyte response were examined for correlation with severity of liver disease. Finally, the effect of the HCV specific CD4+ T lymphocyte response on the effector cells of hepatic fibrosis, hepatic stellate cells, was investigated.

Genomic DNA from CHC patients was tested for the presence of the allele HLA DQB1*0301, a Major Histocompatibility Complex class II molecule which has been shown to be over-represented in individuals with evidence of spontaneous resolution of acute infection. There was no correlation between frequency of this allele in our population and severity of CHC related liver disease. However, analysis of clinical information gathered from these patients confirmed that age, duration of infection and excess alcohol intake were all risk factors for more severe liver disease.  In addition, severity of fibrosis correlated positively with severity of inflammation; supporting the hypothesis that fibrosis is the result of chronic inflammation of CHC.

Functional studies of the CD4+ T lymphocyte response in HCV infection confirmed that HCV lymphocyte proliferative responses are different between CHC patients and those with evidence of spontaneous resolution of HCV infection. There were no differences in the HCV specific proliferative responses between groups of CHC patients discordant for severity of liver disease. However, HCV specific Interferon-γ secretion was more commonly seen in patients with severe liver disease.  CD4+ T lymphocyte responses to non-HCV recall antigens were identical between all groups.

Text
894399.pdf - Version of Record
Available under License University of Southampton Thesis Licence.
Download (22MB)

More information

Published date: 2002

Identifiers

Local EPrints ID: 464925
URI: http://eprints.soton.ac.uk/id/eprint/464925
PURE UUID: 9fcbd4fb-4e28-4b07-ba2f-7cfa797c0a70

Catalogue record

Date deposited: 05 Jul 2022 00:11
Last modified: 23 Jul 2022 02:15

Export record

Contributors

Author: Corinne Brooks

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×