Development of drought resistant varieties of rice (Oryza sativa L.) through in vitro hybridization
Development of drought resistant varieties of rice (Oryza sativa L.) through in vitro hybridization
The aim of this study was to identify drought resistant accessions of rice and to investigate suitable methods for transfer of traits or genes in existing high yielding varieties through in vitro fertilization. Fifty-one rice accessions obtained from the Bangladesh Rice Research Institute (BRRI) were used to identify drought resistant accessions. Significant variations were observed in physio-morphological traits, such as drought score, root shoot length, root shoot dry weight, leaf stomata conductance, stomata number and water use efficiency. Most of the traits studied showed a significant correlation (p<0.01 and p<0.05) and some parameters appeared to be interrelated. Cluster analysis on physio-morphological characteristics has produced 4 to 6 discrete groups of the accessions. The analysis revealed that the most promising accessions for drought resistance were at least four and these were found to be in the same group.
To confirm the physio-morphological findings an analysis of 51 accessions using five isozyme systems was carried out. Maximum polymorphism was found to be with the esterase enzyme systems. Cluster analysis on 5-isozyme investigations also revealed 5 major groups. However, both cluster analyses of isozyme and physio-morphological data showed that 2 accessions could be identified as potential drought resistance types from these studies.
In order to transfer desirable traits to high yielding varieties of rice, attempts were made to develop methods for in vitro isolation of sperm and egg cells and their in vitro fertilization. Unfertilized ovules were macerated with different concentrations of cellulose (0.55 to 2.0%) and pectinase (0.55 to 1.50%) to determine the best concentration of enzymes for isolation of egg cells from embryo sacs. The ovules were incubated at 30oC in cell wall degrading maceration media, leading to their disintegration into loose cells. The best yield (35%) of egg cells was achieved when 0.04% (w/v) calcium chloride, 1% (w/v) cellulase and 0.75% pectinase was used.
University of Southampton
Khalequzzaman, Mohammad
d87075b3-1028-43a6-adb9-1dd61e134a8c
2002
Khalequzzaman, Mohammad
d87075b3-1028-43a6-adb9-1dd61e134a8c
Khalequzzaman, Mohammad
(2002)
Development of drought resistant varieties of rice (Oryza sativa L.) through in vitro hybridization.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The aim of this study was to identify drought resistant accessions of rice and to investigate suitable methods for transfer of traits or genes in existing high yielding varieties through in vitro fertilization. Fifty-one rice accessions obtained from the Bangladesh Rice Research Institute (BRRI) were used to identify drought resistant accessions. Significant variations were observed in physio-morphological traits, such as drought score, root shoot length, root shoot dry weight, leaf stomata conductance, stomata number and water use efficiency. Most of the traits studied showed a significant correlation (p<0.01 and p<0.05) and some parameters appeared to be interrelated. Cluster analysis on physio-morphological characteristics has produced 4 to 6 discrete groups of the accessions. The analysis revealed that the most promising accessions for drought resistance were at least four and these were found to be in the same group.
To confirm the physio-morphological findings an analysis of 51 accessions using five isozyme systems was carried out. Maximum polymorphism was found to be with the esterase enzyme systems. Cluster analysis on 5-isozyme investigations also revealed 5 major groups. However, both cluster analyses of isozyme and physio-morphological data showed that 2 accessions could be identified as potential drought resistance types from these studies.
In order to transfer desirable traits to high yielding varieties of rice, attempts were made to develop methods for in vitro isolation of sperm and egg cells and their in vitro fertilization. Unfertilized ovules were macerated with different concentrations of cellulose (0.55 to 2.0%) and pectinase (0.55 to 1.50%) to determine the best concentration of enzymes for isolation of egg cells from embryo sacs. The ovules were incubated at 30oC in cell wall degrading maceration media, leading to their disintegration into loose cells. The best yield (35%) of egg cells was achieved when 0.04% (w/v) calcium chloride, 1% (w/v) cellulase and 0.75% pectinase was used.
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Published date: 2002
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Local EPrints ID: 464988
URI: http://eprints.soton.ac.uk/id/eprint/464988
PURE UUID: 14b24837-a40e-40fc-8093-eeb5e807178b
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Date deposited: 05 Jul 2022 00:15
Last modified: 16 Mar 2024 19:52
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Mohammad Khalequzzaman
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