Molecular analysis of nutrient transfer in the host/powdery mildew interaction
Molecular analysis of nutrient transfer in the host/powdery mildew interaction
Powdery mildew fungi are biotrophic pathogens that infect a wide variety of economically important plants. When the fungus invades a plant it forms a complex interface between the host plant and the pathogen known as the haustoria complex. The pathogen acts as an additional sink, competing with host sinks such as roots and flowers, resulting in considerable modification of photoassimilate production and partitioning between the host tissues.
This study investigated some of the factors that may contributed to these changes in the Arabidopsis thaliana/Erysiphe cichoracearum interaction. It was shown that expression of the monosaccharide transporters AtSTP3 and AtSTP4 (Arabidopsis thaliana Sugar Transport Protein 3 and 4) is enhanced in infected leaves as shown by northern analysis and reverse transcriptase-polymerase chain reaction (RT-PCR). AtSTP4 induction in infected leaves was supported further by histochemical staining of Arabidopsis plants transformed with an AtSTP4 promoter-β-glucuronidase (GUS) construct. RT-PCR analysis revealed no induction of AtSTP3 and AtSTP4 expression in the incompatible A. thaliana/Blumeria gramminis f.sp. hordei interaction.
In addition, transcript levels for a cell-wall invertase, Atβfruct1, increased substantially in Arabidopsis during attach by E. cichoracearum, as shown by northern analysis and RT-PCR. Similar RT-PCR studies showed no increase in Atβfruct1 expression in the incompatible A. thaliana/B.graminis f.sp. hordei interaction.
DNA microarray analysis using Arabidopsis leaves infected with E. cichoracearum supports the above findings, and indicates that several more monosaccaride transporters may be involved in response to infection by powdery mildew. This finding supports a co-ordinated response model to pathogen attack, involving intricate intra-family responses of specific membrane transporter and invertase gene isoforms.
University of Southampton
Fotopoulos, Vasileios
f3a676b1-2a42-4093-b35c-da263bc90fbb
2003
Fotopoulos, Vasileios
f3a676b1-2a42-4093-b35c-da263bc90fbb
Fotopoulos, Vasileios
(2003)
Molecular analysis of nutrient transfer in the host/powdery mildew interaction.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Powdery mildew fungi are biotrophic pathogens that infect a wide variety of economically important plants. When the fungus invades a plant it forms a complex interface between the host plant and the pathogen known as the haustoria complex. The pathogen acts as an additional sink, competing with host sinks such as roots and flowers, resulting in considerable modification of photoassimilate production and partitioning between the host tissues.
This study investigated some of the factors that may contributed to these changes in the Arabidopsis thaliana/Erysiphe cichoracearum interaction. It was shown that expression of the monosaccharide transporters AtSTP3 and AtSTP4 (Arabidopsis thaliana Sugar Transport Protein 3 and 4) is enhanced in infected leaves as shown by northern analysis and reverse transcriptase-polymerase chain reaction (RT-PCR). AtSTP4 induction in infected leaves was supported further by histochemical staining of Arabidopsis plants transformed with an AtSTP4 promoter-β-glucuronidase (GUS) construct. RT-PCR analysis revealed no induction of AtSTP3 and AtSTP4 expression in the incompatible A. thaliana/Blumeria gramminis f.sp. hordei interaction.
In addition, transcript levels for a cell-wall invertase, Atβfruct1, increased substantially in Arabidopsis during attach by E. cichoracearum, as shown by northern analysis and RT-PCR. Similar RT-PCR studies showed no increase in Atβfruct1 expression in the incompatible A. thaliana/B.graminis f.sp. hordei interaction.
DNA microarray analysis using Arabidopsis leaves infected with E. cichoracearum supports the above findings, and indicates that several more monosaccaride transporters may be involved in response to infection by powdery mildew. This finding supports a co-ordinated response model to pathogen attack, involving intricate intra-family responses of specific membrane transporter and invertase gene isoforms.
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Published date: 2003
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Local EPrints ID: 465170
URI: http://eprints.soton.ac.uk/id/eprint/465170
PURE UUID: 1199d152-09ef-4799-b4ba-3dc40c3144c1
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Date deposited: 05 Jul 2022 00:27
Last modified: 16 Mar 2024 20:00
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Author:
Vasileios Fotopoulos
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