CLND and in-source CID ESMS : a route to a truly quantitative HPLC detector?

Carmody, Carol Ann (2004) CLND and in-source CID ESMS : a route to a truly quantitative HPLC detector? University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

CLDN has been reported to be the universal quantitative detector for nitrogenous compounds offering selectivity, sensitivity and linearity. For linear nitrogen systems this has been observed to be true. However, it has been reported that compounds, which yield diatomic nitrogen on combustion, e.g. tetrazoles and triazoles, give inconsistent results. To address these issues combinatorial methods have been employed to produce series of non-linear response systems to attempt to ascertain whether there is compound related linearity of response for these subsets and whether there is a set of generic rules that could then be applied across the whole range of sub-libraries.

Mass spectrometry offers a means to determine compound class. A range of libraries were studied using in-source CID to identify fragmentation pathways. Subsequently this information can be used to determine which subsets of rules are to be applied to the nitrogen detector response to ensure a quantitative result. The application of this approach should afford a truly universal HPLC detector for nitrogenous compounds. In-source CID has been targeted as the method of choice since ES is widely used as the method of choice for HTS and adding no additional cost compared with an MSMS approach.

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