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An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome

An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome
An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome
Dinoflagellates are microbial eukaryotes that have exceptionally large nuclear genomes; however, their organelle genomes are small and fragmented and contain fewer genes than those of other eukaryotes. The genus Amoebophrya (Syndiniales) comprises endoparasites with high genetic diversity that can infect other dinoflagellates, such as those forming harmful algal blooms (e.g., Alexandrium). We sequenced the genome (~100 Mb) of Amoebophrya ceratii to investigate the early evolution of genomic characters in dinoflagellates. The A. ceratii genome encodes almost all essential biosynthetic pathways for self-sustaining cellular metabolism, suggesting a limited dependency on its host. Although dinoflagellates are thought to have descended from a photosynthetic ancestor, A. ceratii appears to have completely lost its plastid and nearly all genes of plastid origin. Functional mitochondria persist in all life stages of A. ceratii, but we found no evidence for the presence of a mitochondrial genome. Instead, all mitochondrial proteins appear to be lost or encoded in the A. ceratii nucleus.
2375-2548
John, Uwe
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Lu, Yameng
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Wohlrab, Sylke
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Groth, Marco
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Janouškovec, Jan
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Kohli, Gurjeet S.
226ed146-9cfc-4f64-b5ca-ec24632f5049
Mark, Felix C.
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Bickmeyer, Ulf
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Farhat, Sarah
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Felder, Marius
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Frickenhaus, Stephan
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Guillou, Laure
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Keeling, Patrick J.
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Moustafa, Ahmed
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Porcel, Betina M.
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Valentin, Klaus
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Glöckner, Gernot
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John, Uwe
d0167c9f-8077-49b7-a696-8c61f5700598
Lu, Yameng
606d6a4e-539c-404e-aecd-f65b41489458
Wohlrab, Sylke
aeaac754-88a5-4fed-8135-7f8946200e27
Groth, Marco
0a7ab7e5-62aa-4314-b21d-57716e3c6df9
Janouškovec, Jan
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Kohli, Gurjeet S.
226ed146-9cfc-4f64-b5ca-ec24632f5049
Mark, Felix C.
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Bickmeyer, Ulf
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Farhat, Sarah
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Felder, Marius
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Frickenhaus, Stephan
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Guillou, Laure
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Keeling, Patrick J.
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Moustafa, Ahmed
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Porcel, Betina M.
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Valentin, Klaus
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Glöckner, Gernot
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John, Uwe, Lu, Yameng, Wohlrab, Sylke, Groth, Marco, Janouškovec, Jan, Kohli, Gurjeet S., Mark, Felix C., Bickmeyer, Ulf, Farhat, Sarah, Felder, Marius, Frickenhaus, Stephan, Guillou, Laure, Keeling, Patrick J., Moustafa, Ahmed, Porcel, Betina M., Valentin, Klaus and Glöckner, Gernot (2019) An aerobic eukaryotic parasite with functional mitochondria that likely lacks a mitochondrial genome. Science Advances, 5 (4). (doi:10.1126/sciadv.aav1110).

Record type: Article

Abstract

Dinoflagellates are microbial eukaryotes that have exceptionally large nuclear genomes; however, their organelle genomes are small and fragmented and contain fewer genes than those of other eukaryotes. The genus Amoebophrya (Syndiniales) comprises endoparasites with high genetic diversity that can infect other dinoflagellates, such as those forming harmful algal blooms (e.g., Alexandrium). We sequenced the genome (~100 Mb) of Amoebophrya ceratii to investigate the early evolution of genomic characters in dinoflagellates. The A. ceratii genome encodes almost all essential biosynthetic pathways for self-sustaining cellular metabolism, suggesting a limited dependency on its host. Although dinoflagellates are thought to have descended from a photosynthetic ancestor, A. ceratii appears to have completely lost its plastid and nearly all genes of plastid origin. Functional mitochondria persist in all life stages of A. ceratii, but we found no evidence for the presence of a mitochondrial genome. Instead, all mitochondrial proteins appear to be lost or encoded in the A. ceratii nucleus.

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More information

e-pub ahead of print date: 5 April 2019
Published date: 24 April 2019
Additional Information: Copyright © 2019 The Authors

Identifiers

Local EPrints ID: 465682
URI: http://eprints.soton.ac.uk/id/eprint/465682
ISSN: 2375-2548
PURE UUID: dd420281-a112-477e-94aa-836f10a8d9f0
ORCID for Jan Janouškovec: ORCID iD orcid.org/0000-0001-6547-749X

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Date deposited: 05 Jul 2022 02:33
Last modified: 24 Nov 2022 03:03

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Contributors

Author: Uwe John
Author: Yameng Lu
Author: Sylke Wohlrab
Author: Marco Groth
Author: Jan Janouškovec ORCID iD
Author: Gurjeet S. Kohli
Author: Felix C. Mark
Author: Ulf Bickmeyer
Author: Sarah Farhat
Author: Marius Felder
Author: Stephan Frickenhaus
Author: Laure Guillou
Author: Patrick J. Keeling
Author: Ahmed Moustafa
Author: Betina M. Porcel
Author: Klaus Valentin
Author: Gernot Glöckner

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