Synthesis and screening of biocompatible polymers using a multiparallel approach
Synthesis and screening of biocompatible polymers using a multiparallel approach
The aim of this project was to develop novel biocompatible polymers using a parallel approach for their synthesis and screening. Substantially spherical monodisperse beads having a morphology ideally suited for chromatographic applications were successfully synthesised following a two staged templated ‘seeded polymerisation’ from a number of vinyl monomers. Monodisperse porous polymer beads having a controllable porosity and surface areas of about 10-85m2/g were produced from methyl acrylate. The solvents employed in polymerisation were proven to play an important role in determining the structural properties of the beads, with 1-pentanol being the most efficient solvent for generating beads with high surface area and pore size. Analysis of protein adsorption onto beads was performed by flow cytometry, with multiplexed protein adsorption onto mixtures of polymer beads achieved by mixing beads of various types. Fluorescent quantum dots were successfully embedded into pre-formed polymer beads by swelling and collapsing the matrix, giving fluorescently labelled beads. Beads with varying levels of quantum dot incorporation were mixed and the fluorescence intensity corresponding to each set of beads were distinguished by flow cytometric analysis.
A library of 150 biocompatible linear polymers was synthesised in a high-throughput manner, by reacting mixtures of various carbonyl chlorides with polyallylamine; and the final products were analysed by high-throughput DSC, GPC, wettability as well as high throughput FT-IR. The biocompatibilities of the polymers were evaluated. The leukodepletion abilities of two polymers were found to be better than a current commercially available product; and were correlated to the adsorption of PV-GlcNAc and fibrinogen. Platelet binding on the polymers was found to show a good correlation to the adsorption of PV-GlcNAc and IgG.
University of Southampton
Jose, Ann Jasmine
a0ceca23-c78f-4fa4-8e8c-25d59765de36
2005
Jose, Ann Jasmine
a0ceca23-c78f-4fa4-8e8c-25d59765de36
Jose, Ann Jasmine
(2005)
Synthesis and screening of biocompatible polymers using a multiparallel approach.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The aim of this project was to develop novel biocompatible polymers using a parallel approach for their synthesis and screening. Substantially spherical monodisperse beads having a morphology ideally suited for chromatographic applications were successfully synthesised following a two staged templated ‘seeded polymerisation’ from a number of vinyl monomers. Monodisperse porous polymer beads having a controllable porosity and surface areas of about 10-85m2/g were produced from methyl acrylate. The solvents employed in polymerisation were proven to play an important role in determining the structural properties of the beads, with 1-pentanol being the most efficient solvent for generating beads with high surface area and pore size. Analysis of protein adsorption onto beads was performed by flow cytometry, with multiplexed protein adsorption onto mixtures of polymer beads achieved by mixing beads of various types. Fluorescent quantum dots were successfully embedded into pre-formed polymer beads by swelling and collapsing the matrix, giving fluorescently labelled beads. Beads with varying levels of quantum dot incorporation were mixed and the fluorescence intensity corresponding to each set of beads were distinguished by flow cytometric analysis.
A library of 150 biocompatible linear polymers was synthesised in a high-throughput manner, by reacting mixtures of various carbonyl chlorides with polyallylamine; and the final products were analysed by high-throughput DSC, GPC, wettability as well as high throughput FT-IR. The biocompatibilities of the polymers were evaluated. The leukodepletion abilities of two polymers were found to be better than a current commercially available product; and were correlated to the adsorption of PV-GlcNAc and fibrinogen. Platelet binding on the polymers was found to show a good correlation to the adsorption of PV-GlcNAc and IgG.
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Published date: 2005
Identifiers
Local EPrints ID: 465726
URI: http://eprints.soton.ac.uk/id/eprint/465726
PURE UUID: 977a2758-0de3-41c9-b2fa-49980eb0638e
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Date deposited: 05 Jul 2022 02:47
Last modified: 05 Jul 2022 02:47
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Author:
Ann Jasmine Jose
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