IGFBP-1 production in an explant culture system of human Fallopian tube mucosal cells and influences uopn that production
IGFBP-1 production in an explant culture system of human Fallopian tube mucosal cells and influences uopn that production
The Fallopian tube provides the environment for early embryo growth, a process that is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bio-availability of tubal IGFs is modulated by locally produced IGF-binding protein-1 is not clear. An explant culture system from human Fallopian tube mucosa was therefore developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT-PCR, and IGFBP-1 protein measured by ELISA. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein level. We suggest that progesterone might, therefore, play a role in controlling the bio-availability of IGFs to the embryo during early development within the Fallopian tube.
University of Southampton
2005
Davies, Sallie
(2005)
IGFBP-1 production in an explant culture system of human Fallopian tube mucosal cells and influences uopn that production.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The Fallopian tube provides the environment for early embryo growth, a process that is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bio-availability of tubal IGFs is modulated by locally produced IGF-binding protein-1 is not clear. An explant culture system from human Fallopian tube mucosa was therefore developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT-PCR, and IGFBP-1 protein measured by ELISA. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein level. We suggest that progesterone might, therefore, play a role in controlling the bio-availability of IGFs to the embryo during early development within the Fallopian tube.
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Published date: 2005
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Local EPrints ID: 465898
URI: http://eprints.soton.ac.uk/id/eprint/465898
PURE UUID: 6c2ed589-0169-4b53-9cf6-732ba86f481b
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Date deposited: 05 Jul 2022 03:30
Last modified: 05 Jul 2022 03:30
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Author:
Sallie Davies
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