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Characterising the regulatory mechanism of the global regulator BipA

Characterising the regulatory mechanism of the global regulator BipA
Characterising the regulatory mechanism of the global regulator BipA

Previous studies uncovered a novel GTPase termed BipA that regulates a wide range of cellular processes in bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium. These processes include flagella-mediated cell motility, resistance to a host defence protein, acid toae, capsule synthesis, growth at low temperatures and the characteristic ability of enteropathogenic forms of E. coli to rearrange the actin cytoskeleton of infected host cells. BipA resembles a number of known ribosome binding GTPases and occupies a site on the ribosome that coincides with that of EF-G. Further, its GTPase activity is sensitive to high GDP/GTP ratios, but is stimulated by the presence of 70S ribosomes. These observations raise the possibility that BipA functions as a translator factor and that it interacts with a specific mRNA. revealed The primary aim of this study was to investigate further the relationship of BipA and and a possible regulatory cascade involving the protein. Studies with reporter gene that bipA expression was growth rate-regulated. Maximum fusions transcription occurred in the early-exponential phase of growth, with transcription activity proportional to the richness of the growth medium. These findings prompted suggestions that BipA might share control of critical regulatory targets with the global regulator Fis, which has regulatory and expression profiles similar to BipA. BipA was discovered tb be involved in Fis-mediated changes in DNA topology in response to a number of cellular stresses, as well as in the expression of critical componenets for glucose uptake and utilisation. The results of this study may begin to unravel the mechanism of action of a novel global regulatory protein, BipA. The evidence suggests that BipA provides a regulatory link between nutritional availability and the ability of the bacterial cell to commit itself to a number of energy and resource intensive tasks. The study also introduces the possibility that BipA and Fis may work together in a shared and wide spread regulatory control mechanism.

University of Southampton
Pritchard, John Gareth
42725365-fba4-4a79-8ed5-6e7cb2c3ac65
Pritchard, John Gareth
42725365-fba4-4a79-8ed5-6e7cb2c3ac65

Pritchard, John Gareth (2007) Characterising the regulatory mechanism of the global regulator BipA. University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

Previous studies uncovered a novel GTPase termed BipA that regulates a wide range of cellular processes in bacteria such as Escherichia coli and Salmonella enterica serovar Typhimurium. These processes include flagella-mediated cell motility, resistance to a host defence protein, acid toae, capsule synthesis, growth at low temperatures and the characteristic ability of enteropathogenic forms of E. coli to rearrange the actin cytoskeleton of infected host cells. BipA resembles a number of known ribosome binding GTPases and occupies a site on the ribosome that coincides with that of EF-G. Further, its GTPase activity is sensitive to high GDP/GTP ratios, but is stimulated by the presence of 70S ribosomes. These observations raise the possibility that BipA functions as a translator factor and that it interacts with a specific mRNA. revealed The primary aim of this study was to investigate further the relationship of BipA and and a possible regulatory cascade involving the protein. Studies with reporter gene that bipA expression was growth rate-regulated. Maximum fusions transcription occurred in the early-exponential phase of growth, with transcription activity proportional to the richness of the growth medium. These findings prompted suggestions that BipA might share control of critical regulatory targets with the global regulator Fis, which has regulatory and expression profiles similar to BipA. BipA was discovered tb be involved in Fis-mediated changes in DNA topology in response to a number of cellular stresses, as well as in the expression of critical componenets for glucose uptake and utilisation. The results of this study may begin to unravel the mechanism of action of a novel global regulatory protein, BipA. The evidence suggests that BipA provides a regulatory link between nutritional availability and the ability of the bacterial cell to commit itself to a number of energy and resource intensive tasks. The study also introduces the possibility that BipA and Fis may work together in a shared and wide spread regulatory control mechanism.

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Published date: 2007

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Local EPrints ID: 466269
URI: http://eprints.soton.ac.uk/id/eprint/466269
PURE UUID: 8f0a95f1-b034-4b54-9215-481e9b9b87a6

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Date deposited: 05 Jul 2022 05:00
Last modified: 16 Mar 2024 20:36

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Author: John Gareth Pritchard

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