Do cytokine gene polymorphisms influence the development of primary gastric extra-nodal marginal zone B-cell (MALT) lymphoma subsequent to Helicobacter pylori infection?

North, Helen (2006) Do cytokine gene polymorphisms influence the development of primary gastric extra-nodal marginal zone B-cell (MALT) lymphoma subsequent to Helicobacter pylori infection? University of Southampton, Doctoral Thesis.

Record type: Thesis (Doctoral)

Abstract

In this case-control association study, single nucleotide polymorphisms (SNPs) in cytokine genes were investigated, to determine if host cytokine gene polymorphisms influenced the development of GML following H. pylori infection. In addition, the presence of the t(11;18)(q21;q21) translocation in GML is associated with adverse clinical outcome, therefore these SNPs were assessed to determine if there was an association with the presence or absence of this translocation.

Eighteen SNPs selected from 14 cytokine genes and their promoter regions were selected for the case-control association study; IL1A-889, IL1B-31, IL1B-511, IL1B+3953, IL1RN+2018, IL4-590, IL6-174, IL8-251, IL10-592, IL12B+1188, IL18-137, IL18-607, IFNG+874, IFNGR1-56, TGFB1-509, TNF-308, TNF-376, and LTA+252. The cytokine genotype and haplotype frequencies from 206 primary GML patients were compared with 568 uncomplicated H. pylori positive gastritis controls collected from three European populations, UK, Germany and the Netherlands.

None of the cytokine polymorphisms investigated was significantly associated with the development of GML subsequent to H. pylori infection in all three European populations. In addition, there was no trend towards significance in all three populations, to indicate that any of the SNPs investigated were associated with the development of disease. Therefore, the SNPs investigated do not have a major influence in the development of disease. Therefore, the SNPs investigated do not have a major influence in the development of GML subsequent to H. pylori infection. This indicates that the pro-inflammatory cytokine polymorphisms that favour the development of distal gastric adenocarcinoma do not appear to have a major influence in the development of GML subsequent to H. pylori infection. This might indicate that the aetiology of H. pylori associated gastric adenocarcinoma and GML are different.

Text

1071490.pdf - Version of Record

Available under License University of Southampton Thesis Licence.

Download (4MB)