Characterisation of human foetal femur-derived cells and their potential for bone tissue regeneration
Characterisation of human foetal femur-derived cells and their potential for bone tissue regeneration
Human foetal cells were expanded in basal conditions from explant cultures of femurs from foetal tissue at 8-12 weeks post conception. Foetal-derived cells expressed typical stromal antigens but were negative for embryonic markers. Expression of the stromal antigen STRO-1 was observed in foetal samples expanded in basal conditions. Cell proliferation studies indicated a doubling time comparable to human embryonic cells and shorter than adult-derived populations. Clonal studies were used to demonstrate multilineage differentiation of foetal femur-derived cells and expression of lineage specific markers was observed. Differentiation and proliferation were accelerated in foetal populations compared to adult-derived cells, however loss of expansion potential and marked morphological change within foetal populations was observed after serial passages. Growth and expansion of foetal cells in a serum-free chemically defined medium suggests effective future ex vivo expansion strategies for cell-based tissue regeneration. Human foetal femur-derived cell growth was demonstrated on a variety of biomimetic scaffolds, with in vitro differentiation to the osteogenic lineage and maintained cell viability. Foetal cells favoured growth on scaffolds composed of hydroxyapatite and the biodegradable polymer, poly-lactic acid. Utilising the immunodeficient mouse model, in vivo studies demonstrated survival and expansion of foetal-derived cells and integration within a critical size bone defect.
These studies demonstrate the use of human foetal femur-derived cells as a unique alternate cell source to examine mesenchymal stem cell biology, and warrant further investigation for their use in the development of strategies to restore damaged and diseased skeletal tissue.
University of Southampton
Mirmalek-Sani, Sayed-Hadi
af9188b9-3124-40c0-8cd4-203a0fb490f9
2006
Mirmalek-Sani, Sayed-Hadi
af9188b9-3124-40c0-8cd4-203a0fb490f9
Mirmalek-Sani, Sayed-Hadi
(2006)
Characterisation of human foetal femur-derived cells and their potential for bone tissue regeneration.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Human foetal cells were expanded in basal conditions from explant cultures of femurs from foetal tissue at 8-12 weeks post conception. Foetal-derived cells expressed typical stromal antigens but were negative for embryonic markers. Expression of the stromal antigen STRO-1 was observed in foetal samples expanded in basal conditions. Cell proliferation studies indicated a doubling time comparable to human embryonic cells and shorter than adult-derived populations. Clonal studies were used to demonstrate multilineage differentiation of foetal femur-derived cells and expression of lineage specific markers was observed. Differentiation and proliferation were accelerated in foetal populations compared to adult-derived cells, however loss of expansion potential and marked morphological change within foetal populations was observed after serial passages. Growth and expansion of foetal cells in a serum-free chemically defined medium suggests effective future ex vivo expansion strategies for cell-based tissue regeneration. Human foetal femur-derived cell growth was demonstrated on a variety of biomimetic scaffolds, with in vitro differentiation to the osteogenic lineage and maintained cell viability. Foetal cells favoured growth on scaffolds composed of hydroxyapatite and the biodegradable polymer, poly-lactic acid. Utilising the immunodeficient mouse model, in vivo studies demonstrated survival and expansion of foetal-derived cells and integration within a critical size bone defect.
These studies demonstrate the use of human foetal femur-derived cells as a unique alternate cell source to examine mesenchymal stem cell biology, and warrant further investigation for their use in the development of strategies to restore damaged and diseased skeletal tissue.
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Published date: 2006
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Local EPrints ID: 466276
URI: http://eprints.soton.ac.uk/id/eprint/466276
PURE UUID: 7b64533c-67dd-424c-a965-47b535504331
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Date deposited: 05 Jul 2022 05:01
Last modified: 23 Jul 2022 01:15
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Author:
Sayed-Hadi Mirmalek-Sani
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