The role of membrane proteins in the Erisiphe cichoracearum / Arabidopsis thaliana interaction
The role of membrane proteins in the Erisiphe cichoracearum / Arabidopsis thaliana interaction
This study has investigated the transcriptional response of Arabidopsis thaliana to E. cichoracearum infection, using microarray analysis and quantitative real-time PCR. The focus has been on the role of membrane transporters during the infection process. It has been shown that the response to two different powdery mildews, E. cichoracearum and E. orontii is broadly similar. This study has confirmed that a number of transporter genes are up-regulated in response to powdery mildew infections, as well as several members of the PDF1.2 family of defensins.
Homozygous insertional mutants were isolated in six membrane transporter genes (ACA12, LHT1, GLR1.4, PTR3, ALA10 and At2g18680) and one invertase gene AtcwINV1 showing regulation in response to E. cichoracearum infection.
Mutants in LHT1, AtcwINV1 and GLR1.4 all displayed a difference in growth and development compared to wild-type plants, whereas for the other mutants, no marked differences were observed.
The previously observed increased apoplastic concentrations of amino acids induced by ptr3 mutants do not increase the growth of E. cichoracearum on Arabidopsis, and host systems which scavenge amino acids from the apoplasm probably do not contribute to powdery mildew resistance.
Interestingly, ptr3 mutants did not show increased resistance to E. cichoracearum, as has previously been shown to other types of pathogens (necrotrophs).
In summary, this study demonstrates that several host-specific transporter genes, from a number of different membrane-transporter-gene families are induced as a result of powdery mildew infection. However, the progress of infection of E. cichoracearum is not significantly affected in Arabidopsis insertional mutants for these genes. This highlights the complexity and redundancy in the host response to E. cichoracearum.
University of Southampton
Holmes, Robert John
20f68cb6-b752-4375-afb5-82345c25dc23
2007
Holmes, Robert John
20f68cb6-b752-4375-afb5-82345c25dc23
Holmes, Robert John
(2007)
The role of membrane proteins in the Erisiphe cichoracearum / Arabidopsis thaliana interaction.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
This study has investigated the transcriptional response of Arabidopsis thaliana to E. cichoracearum infection, using microarray analysis and quantitative real-time PCR. The focus has been on the role of membrane transporters during the infection process. It has been shown that the response to two different powdery mildews, E. cichoracearum and E. orontii is broadly similar. This study has confirmed that a number of transporter genes are up-regulated in response to powdery mildew infections, as well as several members of the PDF1.2 family of defensins.
Homozygous insertional mutants were isolated in six membrane transporter genes (ACA12, LHT1, GLR1.4, PTR3, ALA10 and At2g18680) and one invertase gene AtcwINV1 showing regulation in response to E. cichoracearum infection.
Mutants in LHT1, AtcwINV1 and GLR1.4 all displayed a difference in growth and development compared to wild-type plants, whereas for the other mutants, no marked differences were observed.
The previously observed increased apoplastic concentrations of amino acids induced by ptr3 mutants do not increase the growth of E. cichoracearum on Arabidopsis, and host systems which scavenge amino acids from the apoplasm probably do not contribute to powdery mildew resistance.
Interestingly, ptr3 mutants did not show increased resistance to E. cichoracearum, as has previously been shown to other types of pathogens (necrotrophs).
In summary, this study demonstrates that several host-specific transporter genes, from a number of different membrane-transporter-gene families are induced as a result of powdery mildew infection. However, the progress of infection of E. cichoracearum is not significantly affected in Arabidopsis insertional mutants for these genes. This highlights the complexity and redundancy in the host response to E. cichoracearum.
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Published date: 2007
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Local EPrints ID: 466387
URI: http://eprints.soton.ac.uk/id/eprint/466387
PURE UUID: eeb3fd61-db5a-4e6f-acc4-2a2b9f4bcaa4
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Date deposited: 05 Jul 2022 05:13
Last modified: 16 Mar 2024 20:40
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Robert John Holmes
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