Microspheres as carrier systems
Microspheres as carrier systems
Micron and submicron microspheres were successfully synthesised and their cytotoxicity toward various cell lines determined. The influence of parameters such as incubation time, concentration and size of the microspheres on cellular cytotoxicity was evaluated and showed overall little cytotoxicity. The cellular uptake of these beads was studied using carboxyfluorescein labelled beads and it showed efficient uptake at low concentrations, and with short incubation times.
Peptides and enhanced green fluorescent protein were covalently coupled to the microspheres and shown by flow cytometry and fluorescent confocal microscopy that uptake by the cells was fast and efficient proving the potential of the crosslinked aminomethyl polystyrene microspheres as a versatile carrier system.
Enzymatic digestion of an internally quenched fluorescent peptide, covalently bound to the microspheres was successfully attempted. Those microspheres were used to transport a membrane impermeable dye into the intracellular space which was released once internalised, suggesting that the synthesised aminomethyl polystyrene microspheres are a practical delivery device for cellular delivery of normally impermeable compounds followed by their cellular release.
University of Southampton
Muzerelle, Mathilde
f6dc8d6f-997f-45e5-b5a6-ce8228c6c2f7
2005
Muzerelle, Mathilde
f6dc8d6f-997f-45e5-b5a6-ce8228c6c2f7
Muzerelle, Mathilde
(2005)
Microspheres as carrier systems.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Micron and submicron microspheres were successfully synthesised and their cytotoxicity toward various cell lines determined. The influence of parameters such as incubation time, concentration and size of the microspheres on cellular cytotoxicity was evaluated and showed overall little cytotoxicity. The cellular uptake of these beads was studied using carboxyfluorescein labelled beads and it showed efficient uptake at low concentrations, and with short incubation times.
Peptides and enhanced green fluorescent protein were covalently coupled to the microspheres and shown by flow cytometry and fluorescent confocal microscopy that uptake by the cells was fast and efficient proving the potential of the crosslinked aminomethyl polystyrene microspheres as a versatile carrier system.
Enzymatic digestion of an internally quenched fluorescent peptide, covalently bound to the microspheres was successfully attempted. Those microspheres were used to transport a membrane impermeable dye into the intracellular space which was released once internalised, suggesting that the synthesised aminomethyl polystyrene microspheres are a practical delivery device for cellular delivery of normally impermeable compounds followed by their cellular release.
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Published date: 2005
Identifiers
Local EPrints ID: 466429
URI: http://eprints.soton.ac.uk/id/eprint/466429
PURE UUID: 4806c507-0dab-4ac2-b26b-465f0d4c950d
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Date deposited: 05 Jul 2022 05:16
Last modified: 05 Jul 2022 05:16
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Author:
Mathilde Muzerelle
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