Seeding of human bone marrow stromal cells onto bone graft substitutes and allograft : an impaction grafting model
Seeding of human bone marrow stromal cells onto bone graft substitutes and allograft : an impaction grafting model
Aims. This thesis examines the potential of Human Bone Marrow Stromal Cell (HBMSC) fractions to augment bone formation after impaction bone grafting. The project will examine the biological response of marrow derived cells on washed morsellised allograft, Beta - Tricalcium Phosphate (P-TCP) and poly(DL-lactic) acid (PLA) synthetic bone graft substitutes in an impaction grafting model. Study Design. Study I: In - vitro analysis of unselected HBMSC populations seeded onto natural (allograft) and synthetic (P-TCP and PLA) scaffolds after impaction. Study II: Parallel in - vitro and in - vivo analysis of unselected and immunoselected HBMSC populations seeded onto natural (allograft) and synthetic (PLA and P-TCP) scaffolds after impaction. Study III: Proof of concept clinical translation of methods from laboratory to operating theatre utilising autologous HBMSCs seeded onto washed allograft and impacted into two patients with proximal femoral defects.
University of Southampton
Tilley, Simon
cd468907-2c35-4f52-a817-6ad0c7977608
2008
Tilley, Simon
cd468907-2c35-4f52-a817-6ad0c7977608
Tilley, Simon
(2008)
Seeding of human bone marrow stromal cells onto bone graft substitutes and allograft : an impaction grafting model.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
Aims. This thesis examines the potential of Human Bone Marrow Stromal Cell (HBMSC) fractions to augment bone formation after impaction bone grafting. The project will examine the biological response of marrow derived cells on washed morsellised allograft, Beta - Tricalcium Phosphate (P-TCP) and poly(DL-lactic) acid (PLA) synthetic bone graft substitutes in an impaction grafting model. Study Design. Study I: In - vitro analysis of unselected HBMSC populations seeded onto natural (allograft) and synthetic (P-TCP and PLA) scaffolds after impaction. Study II: Parallel in - vitro and in - vivo analysis of unselected and immunoselected HBMSC populations seeded onto natural (allograft) and synthetic (PLA and P-TCP) scaffolds after impaction. Study III: Proof of concept clinical translation of methods from laboratory to operating theatre utilising autologous HBMSCs seeded onto washed allograft and impacted into two patients with proximal femoral defects.
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Published date: 2008
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Local EPrints ID: 466588
URI: http://eprints.soton.ac.uk/id/eprint/466588
PURE UUID: 827d9eff-a3c0-47ce-9f51-26c439202c2b
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Date deposited: 05 Jul 2022 05:54
Last modified: 16 Mar 2024 20:47
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Author:
Simon Tilley
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