Modification of dendritic cell phenotype and function : consequences for allergic asthma
Modification of dendritic cell phenotype and function : consequences for allergic asthma
The initial work aimed to characterise monocyte-derived dendritic cells (Mo-DCs) from normal and asthmatic subjects to identify inherited differences with possible relevance for allergic asthma. It was found that Mo-DCs from asthmatic subjects expressed significantly lower level of CD23 at their surface and produced more IL-6 than Mo-DCs from normal subjects.
A primary screen was then developed to address the hypothesis. The primary screen aimed to identify individual agents found at elevated levels in the asthmatic lung, referred to as allergic mediators, that significantly change the phenotype or function of Mo-DCs. Further in-depth investigation of their effects on Mo-DCs and possible relevance in allergic asthma would then be conducted. The effects of allergic mediators on Mo-DCs from both normal and asthmatic subjects were studied. The experimental approach taken showed that the Mo-DC phenotype and function was significantly changed in response to TNF-α (the positive control), IFN-γ, IL-3, IL-5 and IgE, but not in response to MMIP-1α, histamine, PGD2 and IL-13. Four areas of particular interest were identified. 1) Phenotypically mature IFN-γ treated Mo-DCs failed to significantly enhance T cell proliferation. 2) CD23 disappeared from the surface of Mo-DCs in response to TNF-α, IFN-γ and IgE. 3) IL-3 and IL-5 frequently induced a mature Mo-DC phenotype. 4) Mo-DCs from normal and asthmatic subjects responded differently to allergic mediators.
The work presented in this thesis supports a role for DCs in allergic asthma. Evidence also suggested that DCs from asthmatic subjects are more prone to initiate or perpetuate the chronic allergic inflammation seen in asthmatic airways.
University of Southampton
Cazaly, Angelica Maria
ce0a295c-7d1a-4910-8631-0e4a570aa457
2001
Cazaly, Angelica Maria
ce0a295c-7d1a-4910-8631-0e4a570aa457
Cazaly, Angelica Maria
(2001)
Modification of dendritic cell phenotype and function : consequences for allergic asthma.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The initial work aimed to characterise monocyte-derived dendritic cells (Mo-DCs) from normal and asthmatic subjects to identify inherited differences with possible relevance for allergic asthma. It was found that Mo-DCs from asthmatic subjects expressed significantly lower level of CD23 at their surface and produced more IL-6 than Mo-DCs from normal subjects.
A primary screen was then developed to address the hypothesis. The primary screen aimed to identify individual agents found at elevated levels in the asthmatic lung, referred to as allergic mediators, that significantly change the phenotype or function of Mo-DCs. Further in-depth investigation of their effects on Mo-DCs and possible relevance in allergic asthma would then be conducted. The effects of allergic mediators on Mo-DCs from both normal and asthmatic subjects were studied. The experimental approach taken showed that the Mo-DC phenotype and function was significantly changed in response to TNF-α (the positive control), IFN-γ, IL-3, IL-5 and IgE, but not in response to MMIP-1α, histamine, PGD2 and IL-13. Four areas of particular interest were identified. 1) Phenotypically mature IFN-γ treated Mo-DCs failed to significantly enhance T cell proliferation. 2) CD23 disappeared from the surface of Mo-DCs in response to TNF-α, IFN-γ and IgE. 3) IL-3 and IL-5 frequently induced a mature Mo-DC phenotype. 4) Mo-DCs from normal and asthmatic subjects responded differently to allergic mediators.
The work presented in this thesis supports a role for DCs in allergic asthma. Evidence also suggested that DCs from asthmatic subjects are more prone to initiate or perpetuate the chronic allergic inflammation seen in asthmatic airways.
Text
811139.pdf
- Version of Record
More information
Published date: 2001
Identifiers
Local EPrints ID: 467029
URI: http://eprints.soton.ac.uk/id/eprint/467029
PURE UUID: 48f96469-e0eb-4b49-a0c4-83fe3434a55d
Catalogue record
Date deposited: 05 Jul 2022 08:09
Last modified: 16 Mar 2024 20:56
Export record
Contributors
Author:
Angelica Maria Cazaly
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics