Antibody mediated mucosal defences in the female genital tract
Antibody mediated mucosal defences in the female genital tract
The objective of this study was to investigate antibody mediated protection of a mucosal surfaces of both the upper and lower human female genital tracts. To this end model vaccines were given to volunteer women parenterally, orally, and onto the endocervical mucosa. Mucosal washings were them analysed for vaccine specific antibody responses.
Four weeks prior to total abdominal hysterectomy for dysfunctional uterine bleeding 12 women were vaccinated by intramuscular injection, and 12 women by endocervical vaccine administration with 1 ml. of formaldehyde inactivated Hepatitis A virus adsorbed onto aluminium hydroxide. 17 of the women also received a live attenuated Salmonella typhi (Ty 21a) vaccine orally.
Significant Hepatitis A specific IgA and IgG responses were detected in serum four weeks after vaccination both locally and parenterally. High levels of specific IgG and IgA were found throughout the genital tract but particularly in the vagina, cervix and endometrium. The highest concentration s of specific IgG and IgA were detected in the vagina and cervix following cervical vaccination and these responses were significantly greater than those generated by parenteral vaccination. Similar levels of specific IgG and IgA were found throughout the upper and lower genital tracts, suggesting that IgG is at least as important as IgA in providing humoral immunity at the mucosal surface. Immunocytochemical studies confirmed that immune cell populations capable of both antigen uptake and processing, and local antibody production, were present in the lower genital tract following cervical vaccination.
Oral vaccination is clearly a highly acceptable route for vaccine administration. However, low levels of specific IgG and IgA to Salmonella typhi LPS 09 antigen were detected throughout the genital tract in those women who had seroconverted following oral immunisation.
University of Southampton
Moors, Adam
e1652155-6608-4994-8781-49804e141be3
2000
Moors, Adam
e1652155-6608-4994-8781-49804e141be3
Moors, Adam
(2000)
Antibody mediated mucosal defences in the female genital tract.
University of Southampton, Doctoral Thesis.
Record type:
Thesis
(Doctoral)
Abstract
The objective of this study was to investigate antibody mediated protection of a mucosal surfaces of both the upper and lower human female genital tracts. To this end model vaccines were given to volunteer women parenterally, orally, and onto the endocervical mucosa. Mucosal washings were them analysed for vaccine specific antibody responses.
Four weeks prior to total abdominal hysterectomy for dysfunctional uterine bleeding 12 women were vaccinated by intramuscular injection, and 12 women by endocervical vaccine administration with 1 ml. of formaldehyde inactivated Hepatitis A virus adsorbed onto aluminium hydroxide. 17 of the women also received a live attenuated Salmonella typhi (Ty 21a) vaccine orally.
Significant Hepatitis A specific IgA and IgG responses were detected in serum four weeks after vaccination both locally and parenterally. High levels of specific IgG and IgA were found throughout the genital tract but particularly in the vagina, cervix and endometrium. The highest concentration s of specific IgG and IgA were detected in the vagina and cervix following cervical vaccination and these responses were significantly greater than those generated by parenteral vaccination. Similar levels of specific IgG and IgA were found throughout the upper and lower genital tracts, suggesting that IgG is at least as important as IgA in providing humoral immunity at the mucosal surface. Immunocytochemical studies confirmed that immune cell populations capable of both antigen uptake and processing, and local antibody production, were present in the lower genital tract following cervical vaccination.
Oral vaccination is clearly a highly acceptable route for vaccine administration. However, low levels of specific IgG and IgA to Salmonella typhi LPS 09 antigen were detected throughout the genital tract in those women who had seroconverted following oral immunisation.
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Published date: 2000
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Local EPrints ID: 467159
URI: http://eprints.soton.ac.uk/id/eprint/467159
PURE UUID: 45dc4043-5ad3-4126-8471-d2d736aefa54
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Date deposited: 05 Jul 2022 08:14
Last modified: 16 Mar 2024 21:01
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Author:
Adam Moors
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