Application of quantitative second-harmonic generation microscopy to dynamic conditions
Application of quantitative second-harmonic generation microscopy to dynamic conditions
We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.
2546-2554
Kabir, Mohammad M.
7c603a7c-95d5-4047-b5af-37920f089de6
Inavalli, Veera
db7ab576-a272-4f04-b938-3d1772ffbd01
Lau, Tung-Yuen
f17983a2-30a6-4c5f-84d8-61fde3c52e98
Toussaint, Kimani C.
802ac1ff-b200-40cb-9f31-45d36556b3b7
1 November 2013
Kabir, Mohammad M.
7c603a7c-95d5-4047-b5af-37920f089de6
Inavalli, Veera
db7ab576-a272-4f04-b938-3d1772ffbd01
Lau, Tung-Yuen
f17983a2-30a6-4c5f-84d8-61fde3c52e98
Toussaint, Kimani C.
802ac1ff-b200-40cb-9f31-45d36556b3b7
Kabir, Mohammad M., Inavalli, Veera, Lau, Tung-Yuen and Toussaint, Kimani C.
(2013)
Application of quantitative second-harmonic generation microscopy to dynamic conditions.
Biomedical Optics Express, 4 (11), .
(doi:10.1364/boe.4.002546).
Abstract
We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.
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More information
Accepted/In Press date: 14 October 2013
e-pub ahead of print date: 21 October 2013
Published date: 1 November 2013
Additional Information:
© 2013 Optical Society of America
Identifiers
Local EPrints ID: 467268
URI: http://eprints.soton.ac.uk/id/eprint/467268
ISSN: 2156-7085
PURE UUID: 454510f5-c6d5-4981-8361-d1735b826ecd
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Date deposited: 05 Jul 2022 16:32
Last modified: 17 Mar 2024 04:04
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Contributors
Author:
Mohammad M. Kabir
Author:
Veera Inavalli
Author:
Tung-Yuen Lau
Author:
Kimani C. Toussaint
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