Loss of T cell tolerance in the skin following immunopathology is linked to failed restoration of the dermal niche by recruited macrophages

Accepted/In Press date: 22 April 2022

Published date: 17 May 2022

Additional Information: Funding Information: We thank the UCL Comparative Biology Unit for their support with animal work, and UCL Genomics for performing the RNA sequencing. We also acknowledge the support of the Cancer Institute Translational Technology Platforms, in particular Elena Miranda in the pathology service, and the flow cytometry core facility. We are grateful to Anne Pesenacker and Victoria Male for their critical and constructive reading of the manuscript. This work was funded by a BBSRC project grant ( BB/L001608/1 ) and Royal Free charity funding (award 174418 ) to C.L.B., as well as an MRC PhD studentship ( 1450227 ) to H.C.W. L.J. and M.C. acknowledge support from a Wellcome Trust Clinical Research Training Fellowship WT097941 and additional funding from the Bright Red charity and M.E.P. is supported by a Sir Henry Dale Fellowship from Wellcome Trust , 109377/Z/15/Z . The graphical abstract was created with BioRender.com . Funding Information: We thank the UCL Comparative Biology Unit for their support with animal work, and UCL Genomics for performing the RNA sequencing. We also acknowledge the support of the Cancer Institute Translational Technology Platforms, in particular Elena Miranda in the pathology service, and the flow cytometry core facility. We are grateful to Anne Pesenacker and Victoria Male for their critical and constructive reading of the manuscript. This work was funded by a BBSRC project grant (BB/L001608/1) and Royal Free charity funding (award 174418) to C.L.B. as well as an MRC PhD studentship (1450227) to H.C.W. L.J. and M.C. acknowledge support from a Wellcome Trust Clinical Research Training Fellowship WT097941 and additional funding from the Bright Red charity and M.E.P. is supported by a Sir Henry Dale Fellowship from Wellcome Trust, 109377/Z/15/Z. The graphical abstract was created with BioRender.com. H.C.W. designed and performed the majority of the experiments, analyzed and interpreted the data. J.D. performed the bulk of the transcriptomics analyses and some experiments, interpreted the data, and helped prepare figures. S.H. provided analysis of the comparison of dermal and epidermal monocyte populations for Figure 1 and the associated supplemental data. O.K.A. scored histology slides. S.W. I.R.F. and C.A.T. performed the experiments and were involved in establishing the models used. A.F.V. and M.E.P. provided technical advice for the analysis of the single-cell dataset, and M.E.P. discussed and critiqued the manuscript. L.J. and M.C. provided human NanoString data. C.L.B. conceived and designed experiments, analyzed and interpreted the data, wrote the manuscript, and supervised the project. All authors contributed to reviewing and editing the manuscript. The authors declare no competing interests. Publisher Copyright: © 2022 The Authors

Keywords: CP: immunology, contact hypersensitivity, dermis, disease niche, graft-versus-host disease, macrophages, monocytes, regulatory T cells, skin, tolerance

Local EPrints ID: 467296

URI: http://eprints.soton.ac.uk/id/eprint/467296

DOI: doi:10.1016/j.celrep.2022.110819

ISSN: 2211-1247

PURE UUID: 9be0a3c1-5700-416d-9cd8-c7e8bbcf9b7b

ORCID for Andres Vallejo Pulido: orcid.org/0000-0002-4688-0598

Date deposited: 05 Jul 2022 16:49

Last modified: 17 Mar 2024 03:46