A miniature fiber optic surface plasmon resonance sensor for fast detection of staphylococcal enterotoxin B

Slavík, R., Homola, J. and Brynda, E. (2002) A miniature fiber optic surface plasmon resonance sensor for fast detection of staphylococcal enterotoxin B. Biosensors and Bioelectronics, 17 (6-7), 591-595. (doi:10.1016/S0956-5663(02)00013-1).

Record type: Article

Abstract

A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min.

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More information

Accepted/In Press date: 11 January 2002

e-pub ahead of print date: 16 February 2002

Published date: 26 June 2002

Additional Information: Funding Information: This work was supported by the Grant Agency of the Czech Republic under grants 102/99/0549 and 102/00/1536 and by the Grant Agency of the Academy of Sciences of the Czech Republic under grant A4050006. Copyright © 2002 Elsevier Science B.V. All rights reserved.

Keywords: Biosensor, Foodborne pathogens, Optical fiber sensor, Staphylococcal enterotoxin B, Surface plasmon resonance

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