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Identification of plant-like galactolipids in Chromera velia, a photosynthetic relative of malaria parasites.

Identification of plant-like galactolipids in Chromera velia, a photosynthetic relative of malaria parasites.
Identification of plant-like galactolipids in Chromera velia, a photosynthetic relative of malaria parasites.
Apicomplexa are protist parasites that include Plasmodium spp., the causative agents of malaria, and Toxoplasma gondii, responsible for toxoplasmosis. Most Apicomplexa possess a relict plastid, the apicoplast, which was acquired by secondary endosymbiosis of a red alga. Despite being nonphotosynthetic, the apicoplast is otherwise metabolically similar to algal and plant plastids and is essential for parasite survival. Previous studies of Toxoplasma gondii identified membrane lipids with some structural features of plastid galactolipids, the major plastid lipid class. However, direct evidence for the plant-like enzymes responsible for galactolipid synthesis in Apicomplexan parasites has not been obtained. Chromera velia is an Apicomplexan relative recently discovered in Australian corals. C. velia retains a photosynthetic plastid, providing a unique model to study the evolution of the apicoplast. Here, we report the unambiguous presence of plant-like monogalactosyldiacylglycerol and digalactosyldiacylglycerol in C. velia and localize digalactosyldiacylglycerol to the plastid. We also provide evidence for a plant-like biosynthesis pathway and identify candidate galactosyltranferases responsible for galactolipid synthesis. Our study provides new insights in the evolution of these important enzymes in plastid-containing eukaryotes and will help reconstruct the evolution of glycerolipid metabolism in important parasites such as Plasmodium and Toxoplasma.
1083-351X
29893-29903
Botté, CY
1080a3a7-5c6f-4901-9313-d10f23d931d4
Yamaryo-Botté, Y
e793e98b-a26a-4690-8db1-c6f7df4f3127
Janouskovec, J
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Rupasinghe, T
41dbba21-c236-43cb-950e-d5b39a6ba8d9
Keeling, PJ
fd51c2ef-1daa-442d-b186-71001aa7ca7d
Crellin, P
1533c20a-b17b-4ec7-b0b7-a50783ca8566
Coppel, RL
0f59c826-d981-4604-a446-e706803d6261
Maréchal, E
cff02330-7451-41a4-87ad-0acdc969d350
McConville, MJ
ed6abd3f-ab57-4ced-a741-c34596e7298b
McFadden, GI
c5faee6d-6c74-4bf0-9387-bf30087d3e1e
Botté, CY
1080a3a7-5c6f-4901-9313-d10f23d931d4
Yamaryo-Botté, Y
e793e98b-a26a-4690-8db1-c6f7df4f3127
Janouskovec, J
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Rupasinghe, T
41dbba21-c236-43cb-950e-d5b39a6ba8d9
Keeling, PJ
fd51c2ef-1daa-442d-b186-71001aa7ca7d
Crellin, P
1533c20a-b17b-4ec7-b0b7-a50783ca8566
Coppel, RL
0f59c826-d981-4604-a446-e706803d6261
Maréchal, E
cff02330-7451-41a4-87ad-0acdc969d350
McConville, MJ
ed6abd3f-ab57-4ced-a741-c34596e7298b
McFadden, GI
c5faee6d-6c74-4bf0-9387-bf30087d3e1e

Botté, CY, Yamaryo-Botté, Y, Janouskovec, J, Rupasinghe, T, Keeling, PJ, Crellin, P, Coppel, RL, Maréchal, E, McConville, MJ and McFadden, GI (2011) Identification of plant-like galactolipids in Chromera velia, a photosynthetic relative of malaria parasites. The Journal of Biological Chemistry, 286 (34), 29893-29903. (doi:10.1074/jbc.M111.254979).

Record type: Article

Abstract

Apicomplexa are protist parasites that include Plasmodium spp., the causative agents of malaria, and Toxoplasma gondii, responsible for toxoplasmosis. Most Apicomplexa possess a relict plastid, the apicoplast, which was acquired by secondary endosymbiosis of a red alga. Despite being nonphotosynthetic, the apicoplast is otherwise metabolically similar to algal and plant plastids and is essential for parasite survival. Previous studies of Toxoplasma gondii identified membrane lipids with some structural features of plastid galactolipids, the major plastid lipid class. However, direct evidence for the plant-like enzymes responsible for galactolipid synthesis in Apicomplexan parasites has not been obtained. Chromera velia is an Apicomplexan relative recently discovered in Australian corals. C. velia retains a photosynthetic plastid, providing a unique model to study the evolution of the apicoplast. Here, we report the unambiguous presence of plant-like monogalactosyldiacylglycerol and digalactosyldiacylglycerol in C. velia and localize digalactosyldiacylglycerol to the plastid. We also provide evidence for a plant-like biosynthesis pathway and identify candidate galactosyltranferases responsible for galactolipid synthesis. Our study provides new insights in the evolution of these important enzymes in plastid-containing eukaryotes and will help reconstruct the evolution of glycerolipid metabolism in important parasites such as Plasmodium and Toxoplasma.

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e-pub ahead of print date: 28 June 2011
Published date: 26 August 2011
Additional Information: Copyright © 1969, Elsevier

Identifiers

Local EPrints ID: 467562
URI: http://eprints.soton.ac.uk/id/eprint/467562
ISSN: 1083-351X
PURE UUID: 32959615-072f-4c78-ac46-c1e66e5631e7
ORCID for J Janouskovec: ORCID iD orcid.org/0000-0001-6547-749X

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Date deposited: 13 Jul 2022 17:13
Last modified: 11 Aug 2022 02:05

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Contributors

Author: CY Botté
Author: Y Yamaryo-Botté
Author: J Janouskovec ORCID iD
Author: T Rupasinghe
Author: PJ Keeling
Author: P Crellin
Author: RL Coppel
Author: E Maréchal
Author: MJ McConville
Author: GI McFadden

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