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MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia

MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia
MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia

Microglia are the primary resident innate immune cells of the CNS. They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become the gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy, and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia.

1059-1524
ar99
Hansen, Jan
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Bruckner, Matthias
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Pietrowski, Marie
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Jikeli, Jan
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Plescher, Monika
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Beckert, Hannes
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Schnaars, Mareike
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Fulle, Lorenz
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Reitmeier, Katharina
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Langmann, Thomas
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Forster, Irmgard
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Boche, Delphine
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Petzold, Gabor
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Halle, Annett
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Hansen, Jan
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Bruckner, Matthias
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Pietrowski, Marie
9c97b4b7-86b9-42b7-974c-ee4e25b62ee9
Jikeli, Jan
28b426b1-d5b9-4714-b777-5e222825a138
Plescher, Monika
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Beckert, Hannes
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Schnaars, Mareike
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Fulle, Lorenz
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Reitmeier, Katharina
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Langmann, Thomas
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Forster, Irmgard
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Boche, Delphine
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Petzold, Gabor
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Halle, Annett
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Hansen, Jan, Bruckner, Matthias, Pietrowski, Marie, Jikeli, Jan, Plescher, Monika, Beckert, Hannes, Schnaars, Mareike, Fulle, Lorenz, Reitmeier, Katharina, Langmann, Thomas, Forster, Irmgard, Boche, Delphine, Petzold, Gabor and Halle, Annett (2022) MotiQ: an open-source toolbox to quantify the cell motility and morphology of microglia. Molecular Biology of the Cell, 33 (11), ar99, [E21-11-0585R]. (doi:10.1091/mbc.E21-11-0585).

Record type: Article

Abstract

Microglia are the primary resident innate immune cells of the CNS. They possess branched, motile cell processes that are important for their cellular functions. To study the pathways that control microglial morphology and motility under physiological and disease conditions, it is necessary to quantify microglial morphology and motility precisely and reliably. Several image analysis approaches are available for the quantification of microglial morphology and motility. However, they are either not automated, not freely accessible, and/or limited in the number of morphology and motility parameters that can be assessed. Thus, we have developed MotiQ, an open-source, freely accessible software for automated quantification of microglial motility and morphology. MotiQ allows quantification of a diverse set of cellular motility and morphology parameters, including the parameters that have become the gold standard in the microglia field. We demonstrate that MotiQ can be applied to in vivo, ex vivo, and in vitro data from confocal, epifluorescence, or two-photon microscopy, and we compare its results to other analysis approaches. We suggest MotiQ as a versatile and customizable tool to study microglia.

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Accepted/In Press date: 13 June 2022
e-pub ahead of print date: 22 June 2022
Published date: 15 September 2022
Additional Information: Funding Information: We thank Yvonne Biederbick and Antje Baumgartner for excellent technical assistance. We also thank Luis Alvarez for helping with the visualization of the center of mass displacement over time in 3D. This work was supported by grants from the German Science Foundation (EXC 1023 ImmunoSensation), Helmholtz Impuls-und Vernetzungs-fonds and CoEN 5008. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC2151-390873048. Funding Information: We thank Yvonne Biederbick and Antje Baumgartner for excellent technical assistance. We also thank Luis Alvarez for helping with the visualization of the center of mass displacement over time in 3D. This work was supported by grants from the German Science Foundation (EXC 1023 ImmunoSensation), Helmholtz Impuls- und Vernetzungsfonds and CoEN 5008. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC2151-390873048. Funding Information: Postmortem tissue samples from a nonneurological, nonneuro-pathological 62-yr-old control case patient were obtained from the University Hospital Southampton NHS Foundation Trust as part of the UK Brain Archive Information Network (BRAIN UK), which is funded by the Medical Research Council, and Brain Tumour Research. The use of human tissue was covered by the ethical approval provided by BRAIN UK (Research Ethics Committee South Central Hampshire B, reference 14/SC/0098). Publisher Copyright: © 2022 Hansen et al.

Identifiers

Local EPrints ID: 467665
URI: http://eprints.soton.ac.uk/id/eprint/467665
ISSN: 1059-1524
PURE UUID: 5aa09a99-f296-4986-bd9d-1aeb4d5765f2
ORCID for Delphine Boche: ORCID iD orcid.org/0000-0002-5884-130X

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Date deposited: 18 Jul 2022 18:23
Last modified: 17 Mar 2024 07:23

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Contributors

Author: Jan Hansen
Author: Matthias Bruckner
Author: Marie Pietrowski
Author: Jan Jikeli
Author: Monika Plescher
Author: Hannes Beckert
Author: Mareike Schnaars
Author: Lorenz Fulle
Author: Katharina Reitmeier
Author: Thomas Langmann
Author: Irmgard Forster
Author: Delphine Boche ORCID iD
Author: Gabor Petzold
Author: Annett Halle

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