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Microarchitecture and morphology of bone tissue over a wide range of BV/TV assessed by micro-computed tomography and three different threshold backgrounds

Microarchitecture and morphology of bone tissue over a wide range of BV/TV assessed by micro-computed tomography and three different threshold backgrounds
Microarchitecture and morphology of bone tissue over a wide range of BV/TV assessed by micro-computed tomography and three different threshold backgrounds
The microarchitecture of bone both results from and in turn affects the remodelling process. Bone-specific surface, for instance, is one of these important microarchitectural parameters because remodelling is also considered to be a surface-mediated phenomenon (Berli et al.[1]). An understanding of these structural parameters across the widest possible range of porosity is essential to illuminating how bone reacts to disease, in different skeletal sites and in either its cancellous or cortical forms. 112 samples from an elephant femur were examined by micro-computed tomography (μCT), 31 of which contained both mineralised and demineralised tissue. A critical factor in all scans is setting the correct threshold (with background the surrounding medium) and hence 3 different backgrounds were used: air, water and collagen. The effect of the 3 background thresholds on the physical characteristics of bone (BS/TV, BS/BV, TbSp, TbTh, Dmat, vs BV/TV) was then determined. The results showed that using a threshold set by the collagen background had a profound effect on the histomorphometry bone parameters when assessed by μCT. However, the differences between air and water were not significant, suggesting that comparable data can be produced in a laboratory environment when scanning porous bone samples under either wet or dry conditions– counter to common belief. Determining which is more suitable, air or water, in laboratory and in clinical μCT imaging is important to improve the quality and relevance of biomechanics research. The data with collagen as the threshold were illuminating as they showed that remodelling rates and the relative organic to mineral presence varied with BV/TV, concurring with some other recent studies [2,3,4].
Density, Specific surface, bone, bv/tv, cancellous bone, micro-computed X-ray tomography
1350-4533
Adams, G.J.
316008f6-b3e5-4182-94db-9ad9aee2169a
Cook, Richard
06f8322d-81be-4f82-9326-19e55541c78f
Hutchinson, J.R.
ee7c1d0c-1ff3-4c80-a7ae-679624b847b1
Zioupos, P.
a4a5f513-e3ec-44bd-9427-7bf5bc983448
Adams, G.J.
316008f6-b3e5-4182-94db-9ad9aee2169a
Cook, Richard
06f8322d-81be-4f82-9326-19e55541c78f
Hutchinson, J.R.
ee7c1d0c-1ff3-4c80-a7ae-679624b847b1
Zioupos, P.
a4a5f513-e3ec-44bd-9427-7bf5bc983448

Adams, G.J., Cook, Richard, Hutchinson, J.R. and Zioupos, P. (2022) Microarchitecture and morphology of bone tissue over a wide range of BV/TV assessed by micro-computed tomography and three different threshold backgrounds. Medical Engineering & Physics, 106, [103828]. (doi:10.1016/j.medengphy.2022.103828).

Record type: Article

Abstract

The microarchitecture of bone both results from and in turn affects the remodelling process. Bone-specific surface, for instance, is one of these important microarchitectural parameters because remodelling is also considered to be a surface-mediated phenomenon (Berli et al.[1]). An understanding of these structural parameters across the widest possible range of porosity is essential to illuminating how bone reacts to disease, in different skeletal sites and in either its cancellous or cortical forms. 112 samples from an elephant femur were examined by micro-computed tomography (μCT), 31 of which contained both mineralised and demineralised tissue. A critical factor in all scans is setting the correct threshold (with background the surrounding medium) and hence 3 different backgrounds were used: air, water and collagen. The effect of the 3 background thresholds on the physical characteristics of bone (BS/TV, BS/BV, TbSp, TbTh, Dmat, vs BV/TV) was then determined. The results showed that using a threshold set by the collagen background had a profound effect on the histomorphometry bone parameters when assessed by μCT. However, the differences between air and water were not significant, suggesting that comparable data can be produced in a laboratory environment when scanning porous bone samples under either wet or dry conditions– counter to common belief. Determining which is more suitable, air or water, in laboratory and in clinical μCT imaging is important to improve the quality and relevance of biomechanics research. The data with collagen as the threshold were illuminating as they showed that remodelling rates and the relative organic to mineral presence varied with BV/TV, concurring with some other recent studies [2,3,4].

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Accepted/In Press date: 5 June 2022
e-pub ahead of print date: 7 June 2022
Published date: August 2022
Additional Information: Funding Information: The tests were carried out in the Biomechanics laboratories of the Cranfield Forensic Institute of Cranfield University in Shrivenham, UK. The authors acknowledge the in-kind support of the Cranfield Forensic Institute for providing the lab facilities and the Department of Comparative Biomedical Sciences (RVC) and ZSL Whipsnade Zoo for provision of the specimens. The authors acknowledge the support of the EPSRC (GR/N33225; GR/N33102; GR/M59167) & BBSRC (BB/C516844/1). Data for this manuscript when the article is in print will be available through the Cranfield University CORD data depository and preservation system at https://cranfield.figshare.com, Animal tissue samples were used throughout in this research and the work was approved by the internal institutional boards in the universities involved. The research did not involve live animal testing or euthanasia of animals for the procurement of the samples. Funding Information: The authors acknowledge the support of the EPSRC (GR/N33225; GR/N33102; GR/M59167) & BBSRC (BB/C516844/1). Publisher Copyright: © 2022 The Authors
Keywords: Density, Specific surface, bone, bv/tv, cancellous bone, micro-computed X-ray tomography

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Local EPrints ID: 467689
URI: http://eprints.soton.ac.uk/id/eprint/467689
ISSN: 1350-4533
PURE UUID: ccd4e7fa-6aef-4353-ada7-299aef94c34e
ORCID for Richard Cook: ORCID iD orcid.org/0000-0002-2468-5820

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Date deposited: 19 Jul 2022 16:49
Last modified: 09 Aug 2022 01:44

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Contributors

Author: G.J. Adams
Author: Richard Cook ORCID iD
Author: J.R. Hutchinson
Author: P. Zioupos

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