An evolution of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer
An evolution of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer
HMFG1 and HMFG2 are monoclonal antibodies raised against the human milk fat globule. The immunocytochemical application of these antibodies in the diagnosis and determination of prognosis in breast cancer was investigated. Immunocytochemical techniques were established for the optimal demonstration of the HMFG1 and HMFG2 antigens in cytological and histological specimens of primary and secondary breast carcinoma. The immunohistochemical staining patterns of HMFG1 and HMFG2 in histologically normal, benign and malignant breast tissue were determined. In normal and bengin tissue the staining was extracellular. Staining of malignant breast tumours was variable, both in terms of the relative intensity of stain, either extracellularly or intracellularly, and in the extent of intracellular staining. A classification system was devised to represent these two variables and used to grade the staining patterns in tumour biopsies from both a retrospective series of 37 patients and a prospective series of 115 patients with breast carcinoma. The staining patterns classified in this way were indicative of the state of differentiation of the tissue but not of relapse free survival. The most accurate prognostic indicator in breast cancer is the lymph node status. In 50 cases, a comparison was made of the detection of breast carcinoma metastases in the axillary lymph nodes by routine histological diagnosis and by immunohistochemical staining with a panel of monoclonal antibodies, which included MFG1 and HMFG2. Immunohistochemical staining increased the detection of metastases by 17% . Immunocytochemical staining with HMFG1 and HMFG2 failed to distinguish between needle aspirates of benign and malignant breast tumours. The evaluation of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer has been discussed with respect to the biochemical nature and cellular synthesis of the antigens.
University of Southampton
Berry, Nicola
86ffef0f-1664-4ed3-bcea-9d7aa423aed1
1986
Berry, Nicola
86ffef0f-1664-4ed3-bcea-9d7aa423aed1
Jones, B
3c7d1997-4778-43b1-bfc7-2890dddd49fd
Berry, Nicola
(1986)
An evolution of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer.
University of Southampton, Doctoral Thesis, 229pp.
Record type:
Thesis
(Doctoral)
Abstract
HMFG1 and HMFG2 are monoclonal antibodies raised against the human milk fat globule. The immunocytochemical application of these antibodies in the diagnosis and determination of prognosis in breast cancer was investigated. Immunocytochemical techniques were established for the optimal demonstration of the HMFG1 and HMFG2 antigens in cytological and histological specimens of primary and secondary breast carcinoma. The immunohistochemical staining patterns of HMFG1 and HMFG2 in histologically normal, benign and malignant breast tissue were determined. In normal and bengin tissue the staining was extracellular. Staining of malignant breast tumours was variable, both in terms of the relative intensity of stain, either extracellularly or intracellularly, and in the extent of intracellular staining. A classification system was devised to represent these two variables and used to grade the staining patterns in tumour biopsies from both a retrospective series of 37 patients and a prospective series of 115 patients with breast carcinoma. The staining patterns classified in this way were indicative of the state of differentiation of the tissue but not of relapse free survival. The most accurate prognostic indicator in breast cancer is the lymph node status. In 50 cases, a comparison was made of the detection of breast carcinoma metastases in the axillary lymph nodes by routine histological diagnosis and by immunohistochemical staining with a panel of monoclonal antibodies, which included MFG1 and HMFG2. Immunohistochemical staining increased the detection of metastases by 17% . Immunocytochemical staining with HMFG1 and HMFG2 failed to distinguish between needle aspirates of benign and malignant breast tumours. The evaluation of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer has been discussed with respect to the biochemical nature and cellular synthesis of the antigens.
Text
86108320
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Published date: 1986
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Local EPrints ID: 467883
URI: http://eprints.soton.ac.uk/id/eprint/467883
PURE UUID: b206b06c-63f5-4e58-804c-ab0dbd11b084
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Date deposited: 23 Jul 2022 02:17
Last modified: 16 Mar 2024 21:03
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Contributors
Author:
Nicola Berry
Thesis advisor:
B Jones
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