READ ME File For 'Biochemical Data'

Dataset DOI: 10.5258/SOTON/D1192

ReadMe Author: Josh Prince, University of Southampton

This dataset supports the thesis entitled 'Novel Approaches to Discovering Inhibitors of Essential Bacterial Enzymes'


AWARDED BY: Univeristy of Southampton
DATE OF AWARD: 2020

DESCRIPTION OF THE DATA
Biochemical data collected in support of deposited thesis. 

Melting analysis of truncated tRNA with MBG1 (Figure 2.5.2) - Obtained using a Lightcycler 1.5, see methods 9.4.7. Date collected 19-09-16 
Melting analysis of tRNALeuUAG with MBG1 (Figure 3.4.2) - Obtained using a BioRad CFX touch, see methods 9.5.8. Date collected 02-08-18 
Optimization of DH and MBG1 concentrations (Figure 3.4.4) - Obtained using a BMG plate reader, see methods 9.5.10. Date collected 20-11-18
Optimized time course (Figure 4.2.2) - Obtained using a BMG plate reader, see methods 9.6.2. Date collected 12-06-17
Km of SAM (Figure 4.2.4) - Obtained using a BMG plate reader, see methods 9.6.5. Date collected 28-11-18
Inhibitor Assay screen (Figure 4.4.4) - Obtained using a BMG plate reader, see methods 9.6.5. Date collected 10-12-18
HPLC validation (Figure 4.3.7) -See methods 9.6.3. Date collected 11-12-18


Date that the file was created: Januray, 2020