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The complete mitochondrial genome from an unidentified Phalansterium species

The complete mitochondrial genome from an unidentified Phalansterium species
The complete mitochondrial genome from an unidentified Phalansterium species
We describe the complete sequence and organization of the mitochondrial genome from an unidentified species of Phalansterium. This is the first sequenced mitochondrial genome of a member of Variosea clade(Amoebozoa, Conosa). The sequence was assembled from shotgun reads of DNA from a mixed culture containing the euglenid Monomorphina aenigmatica and an amoebozoan that we demonstrate here is closely related to Phalansterium (in nuclear SSU rRNA phylogenies, it branches between two sequences from described species of Phalansterium).Sequence assembly resulted in two distinct mitochondrial genome types, one fragmented and euglenid-like, and the second a single circular mapping contig of 53,614 bp with an amoebozoan-like set of genes. The Phalansterium sp. mitochondrial genome is gene-rich and densely packed, with a large number of tRNAs and an unusually low ratio of identifiable protein-coding genes to unidentified ORFs. These ORFs potentially encode ribosomal proteins exhibiting a divergent character at the sequence level, and whose identification may be hindered by the presence of RNA editing in Phalansterium mitochondria, as inferred from numerous acceptor stemm is-matches typical of amoebozoan tRNA 5’ editing.
25-32
Pombert, Jean-François
9c69ddd8-9562-4666-83d3-299fc0b79cb3
Smirnov, Alexey
e63a6b3d-94ca-4f6b-9b39-840f362e639b
James, Erick R.
b6b754b7-3e0e-4f59-9878-1b15093c276d
Janouškovec, Jan
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Gray, Michael W.
89bedc0b-6a5f-4071-a53f-915cc9eb4bc3
Keeling, Patrick J.
fd51c2ef-1daa-442d-b186-71001aa7ca7d
Pombert, Jean-François
9c69ddd8-9562-4666-83d3-299fc0b79cb3
Smirnov, Alexey
e63a6b3d-94ca-4f6b-9b39-840f362e639b
James, Erick R.
b6b754b7-3e0e-4f59-9878-1b15093c276d
Janouškovec, Jan
fbaa4a5d-872e-465b-b2c3-bb35df455cc6
Gray, Michael W.
89bedc0b-6a5f-4071-a53f-915cc9eb4bc3
Keeling, Patrick J.
fd51c2ef-1daa-442d-b186-71001aa7ca7d

Pombert, Jean-François, Smirnov, Alexey, James, Erick R., Janouškovec, Jan, Gray, Michael W. and Keeling, Patrick J. (2013) The complete mitochondrial genome from an unidentified Phalansterium species. Protist Genomics, 1, 25-32. (doi:10.2478/prge-2013-0002).

Record type: Article

Abstract

We describe the complete sequence and organization of the mitochondrial genome from an unidentified species of Phalansterium. This is the first sequenced mitochondrial genome of a member of Variosea clade(Amoebozoa, Conosa). The sequence was assembled from shotgun reads of DNA from a mixed culture containing the euglenid Monomorphina aenigmatica and an amoebozoan that we demonstrate here is closely related to Phalansterium (in nuclear SSU rRNA phylogenies, it branches between two sequences from described species of Phalansterium).Sequence assembly resulted in two distinct mitochondrial genome types, one fragmented and euglenid-like, and the second a single circular mapping contig of 53,614 bp with an amoebozoan-like set of genes. The Phalansterium sp. mitochondrial genome is gene-rich and densely packed, with a large number of tRNAs and an unusually low ratio of identifiable protein-coding genes to unidentified ORFs. These ORFs potentially encode ribosomal proteins exhibiting a divergent character at the sequence level, and whose identification may be hindered by the presence of RNA editing in Phalansterium mitochondria, as inferred from numerous acceptor stemm is-matches typical of amoebozoan tRNA 5’ editing.

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More information

Accepted/In Press date: 16 August 2013
Published date: 20 August 2013
Additional Information: ©2013 Versita Sp. z o.o.

Identifiers

Local EPrints ID: 468050
URI: http://eprints.soton.ac.uk/id/eprint/468050
PURE UUID: 011c3e57-a3c0-4d52-861f-b29e01e8e394
ORCID for Jan Janouškovec: ORCID iD orcid.org/0000-0001-6547-749X

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Date deposited: 28 Jul 2022 18:50
Last modified: 29 Jul 2022 02:13

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Contributors

Author: Jean-François Pombert
Author: Alexey Smirnov
Author: Erick R. James
Author: Jan Janouškovec ORCID iD
Author: Michael W. Gray
Author: Patrick J. Keeling

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